Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,295,879	T→A	Q603L (CAG→CTG)	h16_B2042 ←	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,295,879	0	T	A	100.0%	21.2 / NA	7	Q603L (CAG→CTG)	h16_B2042	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/2);  total (5/2)

GGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGGC  >  AM260480/2295788‑2296008                                                                                            |                                                                                                                                  ggCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCt                                                                                         >  2:526412/1‑134 (MQ=255)                    tcctGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCccgccg                                                                      >  2:22270/1‑134 (MQ=255)                                                 cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCa                                                                                      <  1:1055822/89‑1 (MQ=255)                                                 cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCa                                                                                      >  2:1055822/1‑89 (MQ=255)                                                      gctgctgctCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTgc                                                                              >  1:1037961/1‑92 (MQ=255)                                                      gctgctgctCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTgc                                                                              <  2:1037961/92‑1 (MQ=255)                                                               cgcgCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCcaac                            >  2:311454/1‑133 (MQ=255)                                                                                        ctgcagctCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGgc  <  1:311454/134‑1 (MQ=255)                                                                                            |                                                                                                                                  GGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGGC  >  AM260480/2295788‑2296008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑