Predicted mutation
evidence seq id position mutation annotation gene description
RA AY305378 682 C→A P190T (CCG→ACG)  hoxK → membrane‑bound [NiFe] hydrogenase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AY3053786820CA100.0% 101.7 / NA 27P190T (CCG→ACG) hoxKmembrane‑bound [NiFe] hydrogenase small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/12);  total (15/12)

CAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCCCGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCACGA  >  AY305378/552‑806
                                                                                                                                  |                                                                                                                            
cAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACgg                                                                                                                           >  2:103/1‑134 (MQ=255)
                  tATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGcc                                                                                                          >  2:586862/1‑133 (MQ=255)
                           gggTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtgatg                                                                                                 <  1:586862/133‑1 (MQ=255)
                             gTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtgatg                                                                                                 >  1:117654/1‑131 (MQ=255)
                             gTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtgatg                                                                                                 <  2:117654/131‑1 (MQ=255)
                                    cgcATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtga                                                                                                   <  2:556319/122‑1 (MQ=255)
                                    cgcATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtga                                                                                                   >  1:556319/1‑122 (MQ=255)
                                                       caggcagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCg                                                                                                                >  2:753180/1‑90 (MQ=255)
                                                       caggcagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCg                                                                                                                <  1:753180/90‑1 (MQ=255)
                                                           cagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGAt                                                                <  2:609897/134‑1 (MQ=255)
                                                           cagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGAt                                                                >  1:609897/1‑134 (MQ=255)
                                                                aaaCCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGGTCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTAt                                                           >  2:1045708/1‑134 (MQ=255)
                                                                   ccTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTcc                                                        <  2:856787/134‑1 (MQ=255)
                                                                                   ccACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGt                                                             <  1:965120/113‑1 (MQ=255)
                                                                                   ccACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGt                                                             >  2:965120/1‑113 (MQ=255)
                                                                                         cGGTTCACAAGGTGGTCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCgg                                  <  1:1045708/134‑1 (MQ=255)
                                                                                           gTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGcc                                >  2:677117/1‑134 (MQ=255)
                                                                                                caAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACa                                         >  1:659010/1‑120 (MQ=255)
                                                                                                caAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACa                                         <  2:659010/120‑1 (MQ=255)
                                                                                                caAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCg                               >  2:357480/1‑130 (MQ=255)
                                                                                                  aGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATg                         <  1:357480/134‑1 (MQ=255)
                                                                                                          aCCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAg                 >  1:496653/1‑134 (MQ=255)
                                                                                                                  gCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCa         >  1:180808/1‑134 (MQ=255)
                                                                                                                     gATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATcc      <  1:103/134‑1 (MQ=255)
                                                                                                                     gATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATcc      <  2:180808/134‑1 (MQ=255)
                                                                                                                      aTTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCa     >  1:1102264/1‑134 (MQ=255)
                                                                                                                          tCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCACGa  >  1:1047331/1‑133 (MQ=255)
                                                                                                                                  |                                                                                                                            
CAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCCCGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCACGA  >  AY305378/552‑806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑