Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	564,366	C→G	P73A (CCC→GCC)	h16_A0537 →	Uncharacterized protein conserved in bacteria

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	564,366	0	C	G	100.0%	16.8 / NA	6	P73A (CCC→GCC)	h16_A0537	Uncharacterized protein conserved in bacteria
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (4/2);  total (4/2)

GTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGT  >  AM260479/564273‑564496                                                                                              |                                                                                                                                   gTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCCGCGGGCCAGCATTGCCGCCGGa                                                                                            >  2:648544/1‑134 (MQ=255) gTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGa                                                                                            >  2:112504/1‑134 (MQ=255)                 aGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTg                                                                            <  1:112504/134‑1 (MQ=255)                                                                                    cTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGTGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcgc         >  2:4132/1‑134 (MQ=255)                                                                                        cgcCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGTGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcgcgcgc     <  1:4132/134‑1 (MQ=255)                                                                                           cAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGt  >  2:1116650/1‑134 (MQ=255)                                                                                              |                                                                                                                                   GTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGCGGT  >  AM260479/564273‑564496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑