Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	160,041	G→C	G102A (GGG→GCG)	h16_A0146 →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	160,041	0	G	C	100.0%	64.5 / NA	18	G102A (GGG→GCG)	h16_A0146	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/8);  total (10/8)

CAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCCG  >  AM260479/159933‑160164                                                                                                             |                                                                                                                             cAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgac                                                                                                     >  2:426187/1‑134 (MQ=255)   gggCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgacga                                                                                                   >  1:1034120/1‑134 (MQ=255)           ccGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTAc                                                                                           >  1:1184036/1‑134 (MQ=255)             gagaTGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGc                                                                                         <  1:426187/134‑1 (MQ=255)                       cGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGt                                                                                >  1:1146139/1‑133 (MQ=255)                        gggCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTc                                                                               <  1:653087/133‑1 (MQ=255)                            aGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACgg                                                                          <  2:1146139/134‑1 (MQ=255)                             gTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGc                                                                         >  2:71077/1‑134 (MQ=255)                                           aGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGa                                                           <  1:71077/134‑1 (MQ=255)                                                 gCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAAc                                                                <  1:388601/123‑1 (MQ=255)                                                 gCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAAc                                                                >  2:388601/1‑123 (MQ=255)                                                   cAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGa                                                   >  2:1163236/1‑134 (MQ=255)                                                                        gcCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCa                              <  2:1034120/134‑1 (MQ=255)                                                                                gcggGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgacga                                                                                                   <  1:145143/57‑1 (MQ=37)                                                                                gcggGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgacga                                                                                                   >  2:145143/1‑57 (MQ=25)                                                                                          gggCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCgg            >  1:272395/1‑134 (MQ=255)                                                                                               gcgTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGaa       <  1:1163236/134‑1 (MQ=255)                                                                                                    gATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGTGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCcg  >  1:1017455/1‑134 (MQ=255)                                                                                                             |                                                                                                                             CAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCCG  >  AM260479/159933‑160164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑