Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 582,759 G→T C352F (TGT→TTT)  h16_A0555 → zinc metalloprotease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479582,7590GT100.0% 166.1 / NA 44C352F (TGT→TTT) h16_A0555zinc metalloprotease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/22);  total (22/22)

CGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGG  >  AM260479/582636‑582882
                                                                                                                           |                                                                                                                           
cgGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATg                                                                                                                   >  1:1021106/1‑134 (MQ=255)
      aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             <  1:730952/134‑1 (MQ=255)
                      tgtgCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAAt                                                                                             <  1:265317/134‑1 (MQ=255)
                            gACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGc                                                                                       <  2:53091/134‑1 (MQ=255)
                                    cGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGcgcg                                                                                                                       >  2:1159858/1‑94 (MQ=255)
                                    cGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGcgcg                                                                                                                       <  1:1159858/94‑1 (MQ=255)
                                             ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCcgc                                                                                                          <  1:612542/98‑1 (MQ=255)
                                             ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCcgc                                                                                                          >  2:612542/1‑98 (MQ=255)
                                               gAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             >  2:204546/1‑93 (MQ=255)
                                               gAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             <  1:204546/93‑1 (MQ=255)
                                                         ccGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             >  1:1130521/1‑83 (MQ=255)
                                                         ccGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             <  2:1130521/83‑1 (MQ=255)
                                                         ccGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCgcggcg                                                          >  2:649046/1‑134 (MQ=255)
                                                           gACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGa                                                                                >  1:336250/1‑110 (MQ=255)
                                                           gACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGa                                                                                <  2:336250/110‑1 (MQ=255)
                                                            aCATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTg                                                       <  1:649046/134‑1 (MQ=255)
                                                                       cgcgTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACt                                            <  2:1021106/134‑1 (MQ=255)
                                                                           tCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             <  1:258223/65‑1 (MQ=255)
                                                                           tCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             >  2:258223/1‑65 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGc                                                                                                                  >  2:919097/1‑58 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGc                                                                                                                  <  1:919097/58‑1 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             <  1:180315/63‑1 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc                                                                                                             >  2:180315/1‑63 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGaa                                                                                              >  2:195238/1‑78 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGaa                                                                                              <  1:195238/78‑1 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc                                                                                     >  2:390002/1‑87 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc                                                                                     <  1:390002/87‑1 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGc                                          <  1:89209/130‑1 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGc                                          >  2:89209/1‑130 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCgg                                       >  1:1120690/1‑133 (MQ=255)
                                                                             aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCgg                                       >  1:1160904/1‑133 (MQ=255)
                                                                                  tGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTg                                                                   <  1:913490/100‑1 (MQ=255)
                                                                                  tGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTg                                                                   >  2:913490/1‑100 (MQ=255)
                                                                                       gCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGcgcgcg                                                             >  2:272869/1‑101 (MQ=255)
                                                                                       gCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGcgcgcg                                                             <  1:272869/101‑1 (MQ=255)
                                                                                       gCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCa                            >  1:709300/1‑134 (MQ=255)
                                                                                            cgcAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTgcg                       >  1:615971/1‑134 (MQ=255)
                                                                                                aGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCt                   <  2:1120690/134‑1 (MQ=255)
                                                                                                     gCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGgc              <  2:615971/134‑1 (MQ=255)
                                                                                                                 gAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACgg  >  1:815390/1‑134 (MQ=255)
                                                                                                                   aTACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCa                                                                                    <  2:238177/50‑1 (MQ=255)
                                                                                                                   aTACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCa                                                                                    >  1:238177/1‑50 (MQ=255)
                                                                                                                   aTACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGc                                          <  2:1159479/92‑1 (MQ=255)
                                                                                                                   aTACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGc                                          >  1:1159479/1‑92 (MQ=255)
                                                                                                                           |                                                                                                                           
CGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGG  >  AM260479/582636‑582882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑