| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 1,076,517 | C→T | P273L (CCG→CTG) | cpaF3 → | flp pilus assembly ATPase CpaF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 1,076,517 | 0 | C | T | 100.0% | 69.7 / NA | 19 | P273L (CCG→CTG) | cpaF3 | flp pilus assembly ATPase CpaF |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/11); total (8/11) | |||||||||||
GTCGGCTCGCTGCTCGACGACATCCTCAAGGAGAGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCCGGGTCCGCTCGAAGAGCCGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTCGCCGGTGATCTTTACCG > AM260479/1076396‑1076618 | gTCGGCTCGCTGCTCGACGACATCCTCAAGGAGAGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGAt < 1:391332/132‑1 (MQ=255) gCTCGCTGCTCGACGACATCCTCAAGGAGAGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGAcgcg < 2:1024146/134‑1 (MQ=255) gagaGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCa < 1:576473/134‑1 (MQ=255) gagaGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCa < 1:68769/134‑1 (MQ=255) tgtTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGAc > 1:687049/1‑131 (MQ=255) gtTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGAc > 2:761779/1‑130 (MQ=255) ttCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCa > 1:540626/1‑125 (MQ=255) ttCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCa < 2:540626/125‑1 (MQ=255) ttCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGAc > 1:131335/1‑129 (MQ=255) agaTCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAgcgc < 2:687049/134‑1 (MQ=255) cTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGAcgcg > 1:1029359/1‑134 (MQ=255) aGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGAt > 2:897397/1‑59 (MQ=255) aGCAGAGCGTCTTTGACGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGAt < 1:897397/59‑1 (MQ=255) tttGACGAAGTGATCAGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAAc < 2:265114/75‑1 (MQ=255) tttGACGAAGTGATCAGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAAc > 1:265114/1‑75 (MQ=255) cGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTCGCCGGTGATCTTTACcg < 2:1094668/134‑1 (MQ=255) cGAAGTGATCGGGCTGGGTCCGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTCGCCGGTGATCTTTACcg < 2:1029359/134‑1 (MQ=255) ccGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTc < 2:427019/98‑1 (MQ=255) ccGCTCGAAGAGCTGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTc > 1:427019/1‑98 (MQ=255) | GTCGGCTCGCTGCTCGACGACATCCTCAAGGAGAGCGTGTTCCGCAGCCAGGAGATCCCGCTGGACGCGCTCAAGCAGAGCGTCTTTGACGAAGTGATCGGGCCGGGTCCGCTCGAAGAGCCGCTGGACGATGACGCGGTCTCGGAAATCATGGTCAACTGCCATGACGAGATCTTTGTCGAGCGCGCCGGCAGGCTGACGCGTTCGCCGGTGATCTTTACCG > AM260479/1076396‑1076618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |