Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,081,530	T→C	L30L (TTG→CTG)	h16_A0990 →	outer membrane protein and related peptidoglycan‑associated (lipo)proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,081,530	0	T	C	100.0%	62.2 / NA	17	L30L (TTG→CTG)	h16_A0990	outer membrane protein and related peptidoglycan‑associated (lipo)proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/7);  total (10/7)

TCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGACAAGGTGCGCCAGGTGGGCGGCATCTAC  >  AM260479/1081447‑1081655                                                                                    |                                                                                                                              tCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                                                   >  2:145112/1‑128 (MQ=255) tCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGCGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                                                   >  1:963927/1‑128 (MQ=255)  cTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                                                   >  2:528940/1‑127 (MQ=255)  cTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                                                   >  1:587808/1‑127 (MQ=255)      gATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCt                                                                        <  1:145112/134‑1 (MQ=255)       aTGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTg                                                                       <  1:483807/134‑1 (MQ=255)             aCCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGcagcag                                                                 <  1:817744/134‑1 (MQ=255)             aCCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGCGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGcagcag                                                                 <  2:963927/134‑1 (MQ=255)              ccTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAg                                                                                            <  1:495965/106‑1 (MQ=255)              ccTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAg                                                                                            >  2:495965/1‑106 (MQ=255)                 gCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCa                                                             >  2:1164893/1‑134 (MQ=255)                                    gATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGccc                                            >  1:137471/1‑132 (MQ=255)                                              tGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGcc                                >  1:433017/1‑134 (MQ=255)                                                              cGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGACAAGGTGCGCCAgg                <  2:1177331/134‑1 (MQ=255)                                                               gTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGcc                                                                                  <  1:840368/67‑1 (MQ=255)                                                               gTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGcc                                                                                  >  2:840368/1‑67 (MQ=255)                                                                             ccTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGAAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGACAAGGTGCGCCAGGTGGGCGGCATCTAc  >  1:731295/1‑133 (MQ=255)                                                                                    |                                                                                                                              TCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGACAAGGTGCGCCAGGTGGGCGGCATCTAC  >  AM260479/1081447‑1081655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑