Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,744,688	G→C	G211A (GGC→GCC)	h16_A1608 →	conserved hypothetical protein containing von Willebrand factor (vWF) type A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,744,688	0	G	C	100.0%	127.0 / NA	33	G211A (GGC→GCC)	h16_A1608	conserved hypothetical protein containing von Willebrand factor (vWF) type A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/15);  total (18/15)

ATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGGCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAGGAC  >  AM260479/1744570‑1744803                                                                                                                       |                                                                                                                    atGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcgcgc                                                                                                      >  2:460115/1‑134 (MQ=255) atGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcgcgc                                                                                                      >  2:138593/1‑134 (MQ=255)            ggggCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGa                                                                                             >  2:1101754/1‑132 (MQ=255)                     cATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGca                                                                                  <  2:785643/134‑1 (MQ=255)                          cacaGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGcagccag                                                                             <  1:138593/134‑1 (MQ=255)                                 gcTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCggtgg                                                                      >  2:865412/1‑134 (MQ=255)                                     cgcACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCt                                                                  <  1:243992/134‑1 (MQ=255)                                            ttGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                           <  1:865412/134‑1 (MQ=255)                                                        tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcgcg                                                                                                       >  2:480873/1‑78 (MQ=255)                                                        tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcgcg                                                                                                       <  1:480873/78‑1 (MQ=255)                                                        tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAg                                               >  1:98287/1‑134 (MQ=255)                                                          gcgcGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAggg                                             >  2:152636/1‑134 (MQ=255)                                                          gcgcGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAggg                                             >  1:727063/1‑134 (MQ=255)                                                                   ccGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTc                                                                                                           <  1:21297/63‑1 (MQ=255)                                                                   ccGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTc                                                                                                           >  2:21297/1‑63 (MQ=255)                                                                   ccGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCt                                    >  2:377452/1‑134 (MQ=255)                                                                    cGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTg                                   <  1:377452/134‑1 (MQ=255)                                                                            ggCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGc                           <  1:152636/134‑1 (MQ=255)                                                                                     cTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAgcgggcgg                  >  1:1027161/1‑134 (MQ=255)                                                                                        ttCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc                 <  2:727063/132‑1 (MQ=255)                                                                                          ccTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATg             >  2:80141/1‑134 (MQ=255)                                                                                            tGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcg                                                                                                         >  1:34135/1‑40 (MQ=38)                                                                                            tGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCcg                                                                                                         <  2:34135/40‑1 (MQ=38)                                                                                                cGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAAc       <  2:1027161/134‑1 (MQ=255)                                                                                                 gACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc                 <  1:1072987/123‑1 (MQ=255)                                                                                                 gACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc                 >  2:1072987/1‑123 (MQ=255)                                                                                                 gACGGCGTAGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGaa        >  1:305620/1‑132 (MQ=255)                                                                                                  aCGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAg     >  2:260918/1‑134 (MQ=255)                                                                                                     gCGTAGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAGGAc  <  2:305620/134‑1 (MQ=255)                                                                                                      cGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc                 <  2:348421/118‑1 (MQ=255)                                                                                                      cGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc                 >  1:348421/1‑118 (MQ=255)                                                                                                         ggACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                           >  1:901163/1‑73 (MQ=255)                                                                                                         ggACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                           <  2:901163/73‑1 (MQ=255)                                                                                                                       |                                                                                                                    ATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGGCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAGGAC  >  AM260479/1744570‑1744803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑