| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 245,288 | T→G | L247V (TTG→GTG) | betA2 → | choline dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 245,288 | 0 | T | G | 100.0% | 102.6 / NA | 27 | L247V (TTG→GTG) | betA2 | choline dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/9); total (18/9) | |||||||||||
GTGCCTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGTTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCACTTGCG > AM260479/245160‑245409 | gTGCCTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGgtggtg < 1:557111/134‑1 (MQ=255) cTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGcgcg < 1:636611/134‑1 (MQ=255) tGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCgg > 2:652339/1‑133 (MQ=255) aacaaGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGc > 2:994743/1‑123 (MQ=255) aacaaGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGc < 1:994743/123‑1 (MQ=255) cgcgACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcg < 2:76606/134‑1 (MQ=255) aCCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcgcgcg > 2:948714/1‑134 (MQ=255) ggccgcaTGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGcgccgc > 1:4313/7‑134 (MQ=255) aCCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcgcc > 2:362876/1‑134 (MQ=255) cGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcgccg > 1:373578/1‑133 (MQ=255) tACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAgc > 2:889239/1‑134 (MQ=255) gCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAgctgc > 2:39643/1‑134 (MQ=255) tGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGgtg > 2:914179/1‑134 (MQ=255) tAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGt < 1:272565/82‑1 (MQ=255) tAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGt > 2:272565/1‑82 (MQ=255) aGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccg < 2:4313/134‑1 (MQ=255) aGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccg > 1:299061/1‑134 (MQ=255) ggCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcgcgcgcgc < 2:586758/63‑1 (MQ=255) ggCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcgcgcgcgc > 1:586758/1‑63 (MQ=255) ggCCGCCATGCGGCCGGGGTGAAGGTGGTGCGCGAGGGCTACCGGAAACCGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccgc < 1:706112/134‑1 (MQ=255) cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCcgcgta > 1:308299/1‑132 (MQ=255) cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCa > 2:395150/1‑134 (MQ=255) cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCa > 1:776817/1‑134 (MQ=255) aTGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCAc > 2:350490/1‑134 (MQ=255) tGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGAc > 2:108643/1‑61 (MQ=255) tGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGAc < 1:108643/61‑1 (MQ=255) cGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCACTTg < 1:914179/134‑1 (MQ=255) gCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCACTTgcg > 1:615233/1‑134 (MQ=255) | GTGCCTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGTTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCACTTGCG > AM260479/245160‑245409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |