| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260480 | 274,470 | (G)10→9 | intergenic (+18/‑626) | h16_B0246 → / → fhaB | Hypothetical protein/filamentous hemagglutinin / adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260480 | 274,461 | 0 | G | . | 88.5% | 86.4 / 6.3 | 26 | intergenic (+9/‑635) | h16_B0246/fhaB | Hypothetical protein/filamentous hemagglutinin / adhesin |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base . (13/10); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGACTT > AM260480/274333‑274589 | gTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCgggggg < 2:2192/134‑1 (MQ=255) cAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGc < 1:491488/134‑1 (MQ=255) tgtgGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGc > 2:357083/1‑134 (MQ=255) aaGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCcgc > 2:137274/1‑134 (MQ=255) tgcttTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCg < 2:980828/112‑1 (MQ=255) tgcttTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCg > 1:980828/1‑112 (MQ=255) gcttTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGcctc > 2:240940/1‑134 (MQ=255) ccTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCgg < 1:240940/133‑1 (MQ=255) cgGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGgcgcg > 2:965037/1‑132 (MQ=255) tGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGAt > 2:1037715/1‑134 (MQ=255) ggCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGc < 1:890666/73‑1 (MQ=255) ggCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGc > 2:890666/1‑73 (MQ=255) gCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa < 1:965037/134‑1 (MQ=255) tgcAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGc > 2:475385/1‑134 (MQ=255) cAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa > 2:1032114/1‑125 (MQ=255) cAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa < 1:1032114/125‑1 (MQ=255) ggTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCg < 1:1037715/134‑1 (MQ=255) gTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGcc > 2:1033968/1‑132 (MQ=255) ttGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCcgc > 1:390679/1‑54 (MQ=255) ttGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCcgc < 2:390679/54‑1 (MQ=255) tgacGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGc > 1:53862/1‑39 (MQ=255) tgacGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGc < 2:53862/39‑1 (MQ=255) tgacGTTTAGC‑‑GGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTc > 2:670082/1‑121 (MQ=255) tgacGTTTAGC‑‑GGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTc < 1:670082/121‑1 (MQ=255) acGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGAtt > 2:244786/1‑76 (MQ=255) acGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGAtt < 1:244786/76‑1 (MQ=255) tttAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGACtt > 2:522495/1‑134 (MQ=255) | GTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGACTT > AM260480/274333‑274589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |