| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 3,515,428 | +C | intergenic (+367/‑456) | tRNA‑Pro (CGG) → / → pilB | tRNA‑Pro/Type IV pilus assembly protein PilB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 3,515,427 | 1 | . | C | 100.0% | 74.0 / NA | 20 | intergenic (+366/‑457) | tRNA‑Pro (CGG)/pilB | tRNA‑Pro/Type IV pilus assembly protein PilB |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (10/10); total (10/10) | |||||||||||
GCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAT > AM260479/3515297‑3515549 | gcggcgGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑cctt < 2:275449/134‑4 (MQ=255) tGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAgg < 1:319430/134‑1 (MQ=255) aaTCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGtct > 2:419240/1‑134 (MQ=255) cttCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGtcttc < 1:419240/129‑1 (MQ=255) ccAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCAt < 2:864497/134‑1 (MQ=255) aaGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATccc < 1:132769/134‑1 (MQ=255) tCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAgg > 2:587516/1‑134 (MQ=255) cTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTg > 1:324622/1‑134 (MQ=255) gCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATcc > 2:74855/1‑105 (MQ=255) gCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATcc < 1:74855/105‑1 (MQ=255) cGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTtgat < 2:324622/134‑1 (MQ=255) tGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTtgatg > 1:250558/1‑133 (MQ=255) aaaCTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑cct > 2:49572/1‑39 (MQ=255) aaaCTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑cct < 1:49572/41‑3 (MQ=255) aTGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAg < 2:250558/134‑1 (MQ=255) cgcTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTgc > 1:803220/1‑107 (MQ=255) cgcTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTgc < 2:803220/107‑1 (MQ=255) cATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCa < 1:862241/47‑1 (MQ=255) cATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCa > 2:862241/1‑47 (MQ=255) cATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATcc > 2:379748/1‑66 (MQ=255) cATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATcc < 1:379748/66‑1 (MQ=255) tCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCa > 1:579145/1‑134 (MQ=255) cTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAt > 2:845685/1‑134 (MQ=255) | GCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAT > AM260479/3515297‑3515549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |