Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	153,689	+G	intergenic (‑67/+93)	h16_B0133 ← / ← h16_B0134	conserved hypothetical protein/outer membrane protein (porin)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	153,687	1	.	G	100.0%	44.3 / NA	13	intergenic (‑65/+95)	h16_B0133/h16_B0134	conserved hypothetical protein/outer membrane protein (porin)
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (6/7);  total (6/7)

GGTGTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑GGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGT  >  AM260480/153566‑153782                                                                                                                           |                                                                                                ggtgTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaa                                                                                      >  2:912101/1‑134 (MQ=255)    gtTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaatga                                                                                   >  2:617749/1‑134 (MQ=255)        gCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                               <  1:617749/134‑1 (MQ=255)        gCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                               <  2:661780/134‑1 (MQ=255)                           cgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATg                                                            <  1:243757/134‑1 (MQ=255)                           cgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATg                                                            <  2:376795/134‑1 (MQ=255)                                  gATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTtc                                                      <  2:938969/133‑1 (MQ=255)                                           cACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGAt                                            >  2:817343/1‑134 (MQ=255)                                              ttCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGAtgtg                                         >  1:441030/1‑134 (MQ=255)                                                      tCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGccc                                 >  1:846341/1‑134 (MQ=255)                                                                            cgcgAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCt           <  1:936747/134‑1 (MQ=255)                                                                                   aGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGaa      >  2:315463/1‑132 (MQ=255)                                                                                     ttCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGt  <  2:441030/134‑1 (MQ=255)                                                                                                                           |                                                                                                GGTGTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑GGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGT  >  AM260480/153566‑153782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑