| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260480 | 1,166,676 | +G | intergenic (+150/‑109) | h16_B1032 → / → h16_B1033 | conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260480 | 1,166,674 | 1 | . | G | 97.4% | 145.3 / ‑3.2 | 39 | intergenic (+148/‑111) | h16_B1032/h16_B1033 | conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (21/17); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACC > AM260480/1166542‑1166799 | aCTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑g < 2:274260/134‑1 (MQ=255) gACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑gggt > 2:811634/1‑132 (MQ=255) ggCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGgtttt < 2:96800/134‑2 (MQ=255) tGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACaacaac < 2:914416/134‑1 (MQ=255) ggCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGt < 1:357302/134‑1 (MQ=255) gCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGt > 2:618516/1‑133 (MQ=255) ggTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGAcg > 1:807565/1‑134 (MQ=255) tCCCGCTCTTTCGCCTTGCTGCCGTGGA‑CCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGt > 1:394466/1‑134 (MQ=255) tctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGAcgcg < 2:854676/128‑1 (MQ=255) tctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGAcgcg > 1:854676/1‑128 (MQ=255) ctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGAt > 2:730540/1‑134 (MQ=255) ctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGAt > 2:448127/1‑134 (MQ=255) tttCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATc > 2:133448/1‑134 (MQ=255) tttCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATc > 2:787520/1‑134 (MQ=255) tttCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATc > 1:661927/1‑134 (MQ=255) gCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑g < 1:325214/58‑1 (MQ=255) gCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑g > 2:325214/1‑58 (MQ=255) ccTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCgag > 1:560475/1‑133 (MQ=255) aCCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACg < 1:787520/134‑1 (MQ=255) ccccAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTAGCGCGAATCGACAACAACTTGGCGTGATCTGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAggctgc > 2:839586/1‑128 (MQ=255) cccAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCaa < 2:560475/134‑1 (MQ=255) gACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGc > 1:1013552/1‑128 (MQ=255) gACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGc < 2:1013552/128‑1 (MQ=255) gACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt < 2:185555/131‑1 (MQ=255) gACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt > 1:185555/1‑131 (MQ=255) ccTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAaga < 2:139869/134‑1 (MQ=255) cAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAgg > 2:178253/1‑133 (MQ=255) aaTTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGt < 1:133448/133‑1 (MQ=255) gCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGa > 2:613502/1‑134 (MQ=255) gtgGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt < 1:752636/116‑1 (MQ=255) gtgGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt > 2:752636/1‑116 (MQ=255) gtgGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACAt > 1:828671/1‑132 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACg < 1:232177/106‑1 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACg > 2:232177/1‑106 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAgg > 2:1003428/1‑131 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAgg < 1:1003428/131‑1 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAg > 2:567969/1‑134 (MQ=255) cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAg > 2:172343/1‑134 (MQ=255) ccGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGaa < 1:741318/133‑1 (MQ=255) ccGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGaa < 2:807565/133‑1 (MQ=255) ccGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGaa < 1:811634/133‑1 (MQ=255) ccGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGaa < 1:172343/133‑1 (MQ=255) cgcgAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAAcc < 1:730540/134‑1 (MQ=255) | ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACC > AM260480/1166542‑1166799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |