Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	1,192,562	+C	coding (163/594 nt)	cccA →	cytochrome c, mono‑or diheme variant

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	1,192,560	1	.	C	100.0%	195.3 / NA	50	V54A (GTC→GCC)	cccA	cytochrome c, mono‑or diheme variant
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/26);  total (24/26)

CCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATG  >  AM260480/1192431‑1192688                                                                                                                                   |                                                                                                                                 ccTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑ccct                                                                                                                              >  2:801930/1‑132 (MQ=255)                cGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc                                                                                                                         >  2:670586/1‑125 (MQ=255)                cGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc                                                                                                                         <  1:670586/125‑1 (MQ=255)                cGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggc                                                                                                                >  2:238446/1‑134 (MQ=255)                cGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggc                                                                                                                >  2:223367/1‑134 (MQ=255)                  gctggctgGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggccg                                                                                                              <  2:485916/134‑1 (MQ=255)                               gCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGc                                                                                                 >  1:841928/1‑134 (MQ=255)                                        gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGccc                                                                                               >  2:530367/1‑127 (MQ=255)                                        gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGccc                                                                                               <  1:828559/127‑1 (MQ=255)                                        gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGccc                                                                                               <  1:530367/127‑1 (MQ=255)                                        gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGccc                                                                                               >  2:828559/1‑127 (MQ=255)                                        gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGa                                                                                        <  1:223367/134‑1 (MQ=255)                                         aaaCAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCGGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc                                                                                       <  1:749962/134‑1 (MQ=255)                                         aaaCAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt                                                                                             >  1:1058121/1‑128 (MQ=255)                                         aaaCAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt                                                                                             <  2:1058121/128‑1 (MQ=255)                                                   aacaacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc                                                                              >  2:1017739/1‑133 (MQ=255)                                                      aacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAgg                                                                          <  1:801930/134‑1 (MQ=255)                                                                      cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc                                                                                                                         >  2:822589/1‑71 (MQ=255)                                                                      cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc                                                                                                                         <  1:822589/71‑1 (MQ=255)                                                                      cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCa                                                              >  2:459521/1‑130 (MQ=255)                                                                      cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCa                                                              <  1:459521/130‑1 (MQ=255)                                                                         tAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCg                                                         <  1:1017739/132‑1 (MQ=255)                                                                               aCATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACcac                                                  >  1:847435/1‑133 (MQ=255)                                                                                cATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACaa                                                >  1:24178/1‑134 (MQ=255)                                                                                  tCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGc                                                          >  1:519718/1‑122 (MQ=255)                                                                                  tCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGc                                                          <  2:519718/122‑1 (MQ=255)                                                                                     aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt                                                                                             <  2:182353/84‑1 (MQ=255)                                                                                     aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt                                                                                             >  1:182353/1‑84 (MQ=255)                                                                                     aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTggg                                           >  1:923461/1‑134 (MQ=255)                                                                                     aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTggg                                           >  1:675068/1‑134 (MQ=255)                                                                                     aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTggg                                           >  1:767706/1‑134 (MQ=255)                                                                                       gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGc                                          >  2:142294/1‑133 (MQ=255)                                                                                       gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGc                                          >  2:303410/1‑133 (MQ=255)                                                                                                  gccgccTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGgcgc                              >  2:123023/1‑134 (MQ=255)                                                                                                   ccgccTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGcc                             <  1:238446/134‑1 (MQ=255)                                                                                                    cgccTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGccc                            <  2:841928/134‑1 (MQ=255)                                                                                                    cgccTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGccc                            <  2:923461/134‑1 (MQ=255)                                                                                                       cTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGa                         <  1:303410/134‑1 (MQ=255)                                                                                                                   cTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTAcc              <  2:24178/133‑1 (MQ=255)                                                                                                                      gcgcTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGgcgc                              <  2:950742/114‑1 (MQ=255)                                                                                                                      gcgcTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGgcgc                              >  1:950742/1‑114 (MQ=255)                                                                                                                      gcgcTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGt            <  1:123023/132‑1 (MQ=255)                                                                                                                       cgcTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAgg                                                                          >  2:589442/1‑69 (MQ=255)                                                                                                                       cgcTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAgg                                                                          <  1:589442/69‑1 (MQ=255)                                                                                                                       cgcTTTGCGCGTCCCTGACCGCCCTCCAGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc                                                                              <  2:677350/65‑1 (MQ=255)                                                                                                                       cgcTTTGCGCGTCCCTGACCGCCCTCCAGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc                                                                              >  1:677350/1‑65 (MQ=255)                                                                                                                         cTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCgg       <  2:767706/134‑1 (MQ=255)                                                                                                                            tGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc                                                                                       <  2:4611/51‑1 (MQ=255)                                                                                                                            tGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc                                                                                       >  1:4611/1‑51 (MQ=255)                                                                                                                            tGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGt      <  2:675068/132‑1 (MQ=255)                                                                                                                              cgcgTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATg  <  1:142294/134‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 CCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATG  >  AM260480/1192431‑1192688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑