| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 1,354,645 | +C | intergenic (+98/‑15) | h16_A1248 → / → h16_A1249 | predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 1,354,643 | 1 | . | C | 97.5% | 149.0 / ‑4.4 | 40 | intergenic (+96/‑17) | h16_A1248/h16_A1249 | predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (24/15); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑G‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGAC > AM260479/1354534‑1354770 | cGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAgg < 1:498825/134‑1 (MQ=255) cgcgcCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAg < 2:551069/134‑1 (MQ=255) aCTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCa > 1:474075/1‑134 (MQ=255) attggacTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCAtt > 2:751118/7‑134 (MQ=255) tCCAAT‑ACCGGGAGCTAAATCAATCAAGACCCGAATTATGAAAACATGGTGCAGGGCATCCACGG‑CCTTGACTTTTTCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt < 1:62676/134‑1 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACttt < 2:712788/72‑1 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACttt < 2:466752/72‑1 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACttt > 1:466752/1‑72 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACttt > 1:712788/1‑72 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAg > 2:952792/1‑90 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAg < 1:952792/90‑1 (MQ=255) aTAACAGGGAGCTAGATCATGCAGGACCGTACTATCGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTg > 1:142669/1‑132 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAg < 1:382326/80‑1 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAg > 2:382326/1‑80 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAg < 2:972339/92‑1 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAg > 1:972339/1‑92 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt < 1:713283/121‑1 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCt > 2:713283/1‑121 (MQ=255) gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCg > 2:790375/1‑131 (MQ=255) aGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGc > 2:573402/1‑134 (MQ=255) ggACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCgg > 1:757317/1‑133 (MQ=255) gTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTc > 1:621864/1‑134 (MQ=255) gTACTATTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTc > 2:79356/1‑134 (MQ=255) aTTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAg > 2:228850/1‑44 (MQ=38) aTTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAg < 1:228850/44‑1 (MQ=38) aTTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAg > 2:166199/1‑51 (MQ=38) aTTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAg < 1:166199/51‑1 (MQ=38) aTTGAAATCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTc > 1:807785/1‑132 (MQ=255) aaaTCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCt < 2:807785/133‑1 (MQ=255) tCATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcg < 1:1072021/134‑1 (MQ=255) cATGTGGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcgg > 1:159925/1‑134 (MQ=255) tgtgGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 2:264681/1‑133 (MQ=255) tgtgGCAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 1:746986/1‑133 (MQ=255) cAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAgc < 2:576700/43‑1 (MQ=38) cAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAgc > 1:576700/1‑43 (MQ=38) cAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGaa > 1:328612/1‑131 (MQ=255) cAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGaa < 2:328612/131‑1 (MQ=255) cAGGCCATCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCa > 2:314690/1‑134 (MQ=255) tCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGac < 1:314690/134‑1 (MQ=255) tCGAC‑GCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGa > 1:99709/1‑133 (MQ=255) | CGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGAC‑G‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGAC > AM260479/1354534‑1354770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |