Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM2604801,017,0680AT28.0% 38.9 / 18.2 25L171Q (CTG→CAG) kdpBpotassium transport system ATPase chain B
Reads supporting (aligned to +/- strand):  ref base A (8/10);  new base T (4/3);  total (12/13)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACTCGCGGATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGCTTCGGCCTTCGGCAAGTGC  >  AM260480/1016940‑1017195
                                                                                                                                |                                                                                                                               
aCTCGCGGATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGcc                                                                                                                            <  2:355763/134‑1 (MQ=255)
    gcgGATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGt                                                                                                                          <  1:251078/132‑1 (MQ=255)
      ggATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCg                                                                                                                      <  1:75788/134‑1 (MQ=255)
      ggATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCg                                                                                                                      <  1:154383/134‑1 (MQ=255)
                         gCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCAc                                                                                                   >  2:938108/1‑134 (MQ=255)
                         gCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCAc                                                                                                   >  1:748329/1‑134 (MQ=255)
                                   gcgcTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCtt                                                                                         <  1:635027/134‑1 (MQ=255)
                                            tcgACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCtt                                                                                >  1:979821/1‑134 (MQ=255)
                                                 cGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGca                                                                           <  1:1013694/134‑1 (MQ=255)
                                                 cGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGca                                                                           <  2:748329/134‑1 (MQ=255)
                                                       cACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTg                                                                     >  2:714446/1‑134 (MQ=255)
                                                       cACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAgc                                                                                                                               >  1:692391/1‑76 (MQ=255)
                                                       cACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAgc                                                                                                                               <  2:692391/76‑1 (MQ=255)
                                                           ccGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGt                                                                 <  1:714446/134‑1 (MQ=255)
                                                                  tCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACggtg                                                          >  2:798282/1‑134 (MQ=255)
                                                                                              gCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCct                              >  1:642645/1‑134 (MQ=255)
                                                                                                  gCCTGTACCAGCACCACGTCGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCctgctg                          >  1:734691/1‑134 (MQ=255)
                                                                                                       tACCAGCACCACGTCGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCtgctt                       <  2:734691/132‑1 (MQ=255)
                                                                                                                cacGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGcttcggcctt            >  1:1028724/1‑134 (MQ=255)
                                                                                                                 acGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGcttcggccttc           <  1:798282/134‑1 (MQ=255)
                                                                                                                     cGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGcc                                                                                      <  2:1013938/55‑1 (MQ=255)
                                                                                                                     cGCCACGGCGCTGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGcc                                                                                      >  1:1013938/1‑55 (MQ=255)
                                                                                                                     cGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGcttcggccttcggc        >  2:497048/1‑133 (MQ=255)
                                                                                                                       ccACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGCTTCGGCCTTCGGCAAg     <  2:229686/134‑1 (MQ=255)
                                                                                                                          cGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGCTTCGGCCTTCGGCAAGTGc  >  2:1053665/1‑134 (MQ=255)
                                                                                                                                |                                                                                                                               
ACTCGCGGATCACCGGCGCCGATTCGCCGGTGATGGCGCTTTCGTCGACCGAGGCCACGCCGTCGATCACTTCGCCGTCGCCGGGGATCATCTCGCCGGCCTGTACCAGCACCACGTCGCCACGGCGCAGCGCCGTGGCGGGGCGCGCTTCGGTGGCACTGCCACGCTTGCCGTCCTTCAGCACACGTGCGGTGACGGTGGTCTTCAGGCCGCGCAGCGCTTCCGCCTGCTGCTTGCTTCGGCCTTCGGCAAGTGC  >  AM260480/1016940‑1017195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑