Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	160,041	G→C	G102A (GGG→GCG)	h16_A0146 →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	160,041	0	G	C	100.0%	12.8 / NA	5	G102A (GGG→GCG)	h16_A0146	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (3/2);  total (3/2)

CCACCAGCCCGCAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCCG  >  AM260479/159922‑160164                                                                                                                        |                                                                                                                             ccaccaGCCCGCAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGGCGGCAGGCCTGGCCGCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGA‑cccgccg                                                                                                               >  1:1032890/1‑129 (MQ=255)                        gagaTGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGc                                                                                         <  2:2019/134‑1 (MQ=255)                                   gggCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGAGTCGATGGCGTGCGCTACGACccgccgccg                                                                                                             <  1:919305/103‑1 (MQ=255)                                   gggCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGAGTCGATGGCGTGCGCTACGACccgccgccg                                                                                                             >  2:919305/1‑103 (MQ=255)                                                                                                               gATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCcg  >  1:965887/1‑134 (MQ=255)                                                                                                                        |                                                                                                                             CCACCAGCCCGCAGGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGACGGCAGCCGCTACCGGCTGGCGGTCGAAACCCG  >  AM260479/159922‑160164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑