Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	293,407	T→A	Y759N (TAC→AAC)	flhF →	Flagellar GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	293,407	0	T	A	100.0%	137.1 / NA	36	Y759N (TAC→AAC)	flhF	Flagellar GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/16);  total (20/16)

CGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGT  >  AM260480/293295‑293528                                                                                                                 |                                                                                                                          cgccTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCAccg                                                                                                      >  2:630498/1‑134 (MQ=255)      tCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTg                                                                                                 >  2:685116/1‑134 (MQ=255)                    gctggcCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                   <  1:685116/134‑1 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCcgc                                                                                                             >  2:740177/1‑81 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCcgc                                                                                                             <  1:740177/81‑1 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                             <  2:783361/97‑1 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                             >  1:783361/1‑97 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                   >  1:327903/1‑107 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                   <  2:327903/107‑1 (MQ=255)                                               ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGc                                                         >  2:1047887/1‑133 (MQ=255)                                                 ggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                      >  2:1037114/1‑134 (MQ=255)                                                 ggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                      >  1:783323/1‑134 (MQ=255)                                                   tgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                    <  1:1037114/134‑1 (MQ=255)                                                            cGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGcc                                            >  1:447746/1‑133 (MQ=255)                                                                   cAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCt                                    >  1:757424/1‑134 (MQ=255)                                                                     aTGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTgc                                  <  1:630498/134‑1 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                             >  2:417287/1‑67 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                             <  1:417287/67‑1 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAg                                                                  >  2:825288/1‑94 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAg                                                                  <  1:825288/94‑1 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                    >  2:643695/1‑108 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                    <  1:643695/108‑1 (MQ=255)                                                                             aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                          >  2:652266/1‑134 (MQ=255)                                                                                  ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                   <  2:203230/72‑1 (MQ=255)                                                                                  ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                   >  1:203230/1‑72 (MQ=255)                                                                                  ccGCATGCTGGCATTGCTAGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                 <  2:1000176/90‑1 (MQ=255)                                                                                  ccGCATGCTGGCATTGCTAGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                 >  1:1000176/1‑90 (MQ=255)                                                                                     cATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGcgcc                  <  2:757424/134‑1 (MQ=255)                                                                                         cTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                    >  1:804371/1‑96 (MQ=255)                                                                                         cTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                    <  2:804371/96‑1 (MQ=255)                                                                                         cTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCcgcg               >  1:667212/1‑133 (MQ=255)                                                                                         cTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCcgcg               >  1:333054/1‑133 (MQ=255)                                                                                            gCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCac            <  1:774430/133‑1 (MQ=255)                                                                                                     gACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGt  <  2:667212/134‑1 (MQ=255)                                                                                                      aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCt                                                                                           <  2:245722/44‑1 (MQ=255)                                                                                                      aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCt                                                                                           >  1:245722/1‑44 (MQ=255)                                                                                                                 |                                                                                                                          CGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGT  >  AM260480/293295‑293528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑