Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	433,189	A→C	R133R (CGA→CGC)	h16_B0381 →	Acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	433,189	0	A	C	100.0%	209.0 / NA	54	R133R (CGA→CGC)	h16_B0381	Acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/27);  total (27/27)

AGATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCCGC  >  AM260480/433059‑433318                                                                                                                                   |                                                                                                                                  aGATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTAc                                                                                                                                >  1:21491/1‑134 (MQ=255)        cGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTAcc                                                                                                                         >  2:426217/1‑134 (MQ=255)           cTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGcc                                                                                                                      >  1:398435/1‑134 (MQ=255)              ggCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCtt                                                                                                                    <  1:426217/133‑1 (MQ=255)              ggCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGGGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTg                                                                                                                   <  2:82310/134‑1 (MQ=255)                 cATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTggg                                                                                                                 >  2:665738/1‑133 (MQ=255)                    gAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACg                                                                                                             >  2:95320/1‑134 (MQ=255)                            tGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                     <  1:1067100/134‑1 (MQ=255)                              aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  2:901730/1‑134 (MQ=255)                                gCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTc                                                                                                  >  1:272597/1‑133 (MQ=255)                                 cTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                                <  1:738099/134‑1 (MQ=255)                                    gcgcATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGAc                                                                                               <  2:664131/132‑1 (MQ=255)                                           aCCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTAc                                                                                      <  2:272597/134‑1 (MQ=255)                                               gctgctTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   <  1:163496/117‑1 (MQ=255)                                               gctgctTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  2:163496/1‑117 (MQ=255)                                                    ttGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACggg                                                                             >  1:200304/1‑134 (MQ=255)                                                          gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTggag                                                                       >  1:1003716/1‑134 (MQ=255)                                                              cTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACg                                                                   <  2:1003716/134‑1 (MQ=255)                                                                 cAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGcc                                                                 <  1:95320/133‑1 (MQ=255)                                                                           gtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCa                                                      <  1:403356/134‑1 (MQ=255)                                                                           gtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCa                                                      <  2:518200/134‑1 (MQ=255)                                                                            tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                                >  2:555729/1‑91 (MQ=255)                                                                            tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                                <  1:555729/91‑1 (MQ=255)                                                                            tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                     <  2:398435/134‑1 (MQ=255)                                                                                gggcggCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGa                                                 <  2:38530/134‑1 (MQ=255)                                                                                  gcggcATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTg                                                                                                                   >  1:981052/1‑66 (MQ=255)                                                                                  gcggcATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTg                                                                                                                   <  2:981052/66‑1 (MQ=255)                                                                                  gcggcATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATg                                               >  2:451903/1‑134 (MQ=255)                                                                                          gAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTa                                                               >  1:691972/1‑110 (MQ=255)                                                                                          gAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTa                                                               <  2:691972/110‑1 (MQ=255)                                                                                           aGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTTATGGGCtactac                                      >  2:222440/1‑134 (MQ=255)                                                                                                    ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTAc                                                                                      <  1:862456/77‑1 (MQ=255)                                                                                                    ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTAc                                                                                      >  2:862456/1‑77 (MQ=255)                                                                                                                   tCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                     <  1:614991/47‑1 (MQ=255)                                                                                                                   tCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                     >  2:614991/1‑47 (MQ=255)                                                                                                                   tCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGgcgcgc              <  1:211972/134‑1 (MQ=255)                                                                                                                        ccGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                       >  1:1015267/1‑40 (MQ=38)                                                                                                                        ccGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                       <  2:1015267/40‑1 (MQ=38)                                                                                                                        ccGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCAt         >  1:397006/1‑134 (MQ=255)                                                                                                                         cgcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCt                                                                           >  2:215917/1‑67 (MQ=255)                                                                                                                         cgcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCt                                                                           <  1:215917/67‑1 (MQ=255)                                                                                                                          gcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   <  2:346856/42‑1 (MQ=255)                                                                                                                          gcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  1:346856/1‑42 (MQ=255)                                                                                                                           cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTggag                                                                       >  1:112859/1‑69 (MQ=255)                                                                                                                           cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTggag                                                                       <  2:112859/69‑1 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACt                                                                                     <  2:983609/51‑1 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACt                                                                                     >  1:983609/1‑51 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTAc                                                              <  2:1025313/74‑1 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTAc                                                              >  1:1025313/1‑74 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGctac                                         <  1:27392/95‑1 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGctac                                         >  2:27392/1‑95 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGc     <  1:255678/131‑1 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGc     >  2:255678/1‑131 (MQ=255)                                                                                                                               gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCcgc  >  2:682110/1‑134 (MQ=255)                                                                                                                                   |                                                                                                                                  AGATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCCGC  >  AM260480/433059‑433318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑