Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,295,879	T→A	Q603L (CAG→CTG)	h16_B2042 ←	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,295,879	0	T	A	100.0%	54.0 / NA	15	Q603L (CAG→CTG)	h16_B2042	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/7);  total (8/7)

GCGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGG  >  AM260480/2295758‑2295992                                                                                                                          |                                                                                                                  gcgGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATctgc                                                                                                        <  2:612815/133‑1 (MQ=255)                ccACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAAcc                                                                                         <  1:282495/133‑1 (MQ=255)                   cTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACctgct                                                                                     >  2:434515/1‑134 (MQ=255)                        gTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGt                                                                                <  1:434515/134‑1 (MQ=255)                              gggCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCgcgc                                                                          >  2:971610/1‑134 (MQ=255)                                  gTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCa                                                                      >  1:755412/1‑134 (MQ=255)                                    cgcgTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATg                                                                    >  1:22376/1‑134 (MQ=255)                                          gCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTgc                                                              >  2:164618/1‑134 (MQ=255)                                                       ctgTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCg                                                 >  2:96083/1‑134 (MQ=255)                                                          ttGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGt                                              <  2:22376/134‑1 (MQ=255)                                                                     gATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCg                                   >  2:376689/1‑134 (MQ=255)                                                                               cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCgcccggctggc                         <  1:250575/134‑1 (MQ=255)                                                                               cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCgcccggctggc                         <  1:459472/134‑1 (MQ=255)                                                                               cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCgcccggctggc                         <  1:971610/134‑1 (MQ=255)                                                                                                      cgcgcTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCgg  >  1:137467/1‑134 (MQ=255)                                                                                                                          |                                                                                                                  GCGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGG  >  AM260480/2295758‑2295992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑