Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,737,510	T→G	intergenic (‑227/+17)	h16_B2423 ← / ← h16_B2424	periplasmic solute binding protein/Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,737,510	0	T	G	100.0%	38.8 / NA	12	intergenic (‑227/+17)	h16_B2423/h16_B2424	periplasmic solute binding protein/Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/8);  total (4/8)

GCATGGCCAGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAACGACAATGGCTTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGCCAACGGCAATGGCGGGCGCCTGGGTGGCGGCGGGCAGCGTGGCAGTGGCCGGGGCCGCGGG  >  AM260480/2737396‑2737625                                                                                                                   |                                                                                                                    gcATGGCCAGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAg                                                                                                 >  1:527102/1‑134 (MQ=255)         aGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTc                                                                                           >  2:826132/1‑132 (MQ=255)         aGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGa                                                                                         <  1:133669/134‑1 (MQ=255)         aGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGa                                                                                         <  1:586728/134‑1 (MQ=255)         aGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGa                                                                                         <  2:527102/134‑1 (MQ=255)          cacgccggcAGGGCAAGCGTGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCg                                                                                          <  1:829631/129‑1 (MQ=255)                         aGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATG‑TTCAGGGAGTCGAGGGCGCGCCTGGAGCcg                                                                        <  2:1079213/134‑1 (MQ=255)                                   ccGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGc                                                               >  2:831642/1‑134 (MQ=255)                                        aGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGCCAACg                                                          <  2:334648/134‑1 (MQ=255)                                                                     gcaacacgcgcgGCGGGAATCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGAGGGTGCGCCTGGAGCCGCGGCAAGGCCAACGGCAATGGCGGGCGCCTGGGTGGCGGCggg                             >  1:1045232/7‑134 (MQ=255)                                                                                       aTCAGGGACGGGAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGCCAACGGCAATGGCGGGCGCCTGGGTGGCGGCGGGCAGCGTGGCAGTGGCCgg           <  2:294507/134‑1 (MQ=255)                                                                                                gggAGTCAGCGAC‑ATGGCGTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGCCAACGGCAATGGCGGGCGCCTGGGTGGCGGCGGGCAGCGTGGCAGTGGCCGGGGCCGCggg  <  2:1045232/134‑1 (MQ=255)                                                                                                                   |                                                                                                                    GCATGGCCAGCGGCCGGCAGGGCAAGCGCGGCGCCCGCGAGGGCGCGCGCCAGCCAGCGCCACGCGCGTGTTGCCGCGCGGCGGGAATCAGGGACGGGAGTCAACGACAATGGCTTCAAACGGTTCATGTTTCAGGGAGTCGAGGGCGCGCCTGGAGCCGCGGCAAGGCCAACGGCAATGGCGGGCGCCTGGGTGGCGGCGGGCAGCGTGGCAGTGGCCGGGGCCGCGGG  >  AM260480/2737396‑2737625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑