Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	564,366	C→G	P73A (CCC→GCC)	h16_A0537 →	Uncharacterized protein conserved in bacteria

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	564,366	0	C	G	100.0%	53.7 / NA	15	P73A (CCC→GCC)	h16_A0537	Uncharacterized protein conserved in bacteria
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/8);  total (7/8)

ACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGC  >  AM260479/564269‑564493                                                                                                  |                                                                                                                                aCCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTgccgc                                                                                             >  2:269772/1‑134 (MQ=255)          ggcggcCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCt                                                                                     <  1:818548/133‑1 (MQ=255)          ggcggcCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCt                                                                                     >  2:818548/1‑133 (MQ=255)           gcggcCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGc                                                                                   <  1:562726/134‑1 (MQ=255)                                   gaggccgcggccgcGGCCGACGGCCACGACCCGCGCGACCACGATGCCGGCGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCCGCGGGCCAGCATTGCCGCCGGGCGCTGCCGCACGCGCGGATGCGGCCCTggg                                                           >  1:612027/3‑132 (MQ=255)                                          cggccgcggccgACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGcgccgc                                                     >  2:401898/1‑133 (MQ=255)                                            gccgcggccgACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGAt                                                  <  2:418298/134‑1 (MQ=255)                                               gcggccgACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATg                                                 >  2:93437/1‑132 (MQ=255)                                                 ggccgACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGc                                             <  1:269772/134‑1 (MQ=255)                                                                                  cGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTg                                     <  1:442633/109‑1 (MQ=255)                                                                                  cGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTg                                     >  2:442633/1‑109 (MQ=255)                                                                                     cGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAAcc         >  2:833946/1‑134 (MQ=255)                                                                                       ccTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcg       <  1:321222/134‑1 (MQ=255)                                                                                       ccTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcg       <  1:833946/134‑1 (MQ=255)                                                                                            cgcCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCcgcgcgc  <  1:833939/134‑1 (MQ=255)                                                                                                  |                                                                                                                                ACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACCCGCGCGC  >  AM260479/564269‑564493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑