Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 2,016,501 T→C V678V (GTT→GTC)  h16_A1857 → Uncharacterized protein involved in exopolysaccharide biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM2604792,016,5010TC100.0% 167.0 / NA 43V678V (GTT→GTC) h16_A1857Uncharacterized protein involved in exopolysaccharide biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

CTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016376‑2016629
                                                                                                                             |                                                                                                                                
ctgcAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCg                                                                                                                           <  1:65204/133‑1 (MQ=255)
      ccAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg                                                                                                                    <  2:637595/134‑1 (MQ=255)
                ccACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  2:798448/134‑1 (MQ=255)
                                  ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  1:630060/1‑128 (MQ=255)
                                       ggCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt                                                                                                                          <  2:946586/95‑1 (MQ=255)
                                       ggCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt                                                                                                                          >  1:946586/1‑95 (MQ=255)
                                                         gtgCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg                                                                                                                         >  2:749908/1‑78 (MQ=255)
                                                         gtgCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg                                                                                                                         <  1:749908/78‑1 (MQ=255)
                                                           gCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGgcg                                                                >  2:59232/1‑133 (MQ=255)
                                                           gCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGgcg                                                                >  1:957240/1‑133 (MQ=255)
                                                              tGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          >  2:849863/1‑88 (MQ=255)
                                                              tGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  1:849863/88‑1 (MQ=255)
                                                                aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt                                                                                                                          <  2:976350/70‑1 (MQ=255)
                                                                aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt                                                                                                                          >  1:976350/1‑70 (MQ=255)
                                                                   gACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGAc                                                       <  2:763594/134‑1 (MQ=255)
                                                                     ccGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGt                                                     >  1:686451/1‑134 (MQ=255)
                                                                        ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc                                                  >  1:461511/1‑134 (MQ=255)
                                                                        ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc                                                  <  2:630060/134‑1 (MQ=255)
                                                                         cATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGcc                                                 <  1:248929/134‑1 (MQ=255)
                                                                            tgctgcCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  1:496840/74‑1 (MQ=255)
                                                                            tgctgcCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  1:481106/74‑1 (MQ=255)
                                                                            tgctgcCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          >  2:481106/1‑74 (MQ=255)
                                                                            tgctgcCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          >  2:496840/1‑74 (MQ=255)
                                                                              ctgcCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACg                                            <  1:658985/134‑1 (MQ=255)
                                                                                 ccGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACgg                                           <  2:461511/132‑1 (MQ=255)
                                                                                               gcTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAAc                            >  1:598705/1‑133 (MQ=255)
                                                                                               gcTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAAc                            >  1:1063272/1‑133 (MQ=255)
                                                                                                    tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACtt                       >  2:364043/1‑133 (MQ=255)
                                                                                                               aTGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           <  2:294949/134‑1 (MQ=255)
                                                                                                                  gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGcc                                                                        >  2:75084/1‑70 (MQ=255)
                                                                                                                  gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGcc                                                                        >  2:224614/1‑70 (MQ=255)
                                                                                                                  gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGcc                                                                        <  1:75084/70‑1 (MQ=255)
                                                                                                                  gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGcc                                                                        <  1:224614/70‑1 (MQ=255)
                                                                                                                  gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCa        >  2:842711/1‑134 (MQ=255)
                                                                                                                   cGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc       <  2:957240/134‑1 (MQ=255)
                                                                                                                    gggCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAcc      <  1:59232/134‑1 (MQ=255)
                                                                                                                       cACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTTATCTTCAACGACTTCACCgg                 >  1:925586/1‑120 (MQ=255)
                                                                                                                       cACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTTATCTTCAACGACTTCACCgg                 <  2:925586/120‑1 (MQ=255)
                                                                                                                         cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGCCTTCACCGGCCACCTGCACCGCTa  >  2:451008/1‑133 (MQ=255)
                                                                                                                         cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           >  2:344757/1‑124 (MQ=255)
                                                                                                                         cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           >  2:25394/1‑124 (MQ=255)
                                                                                                                         cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           <  1:344757/124‑1 (MQ=255)
                                                                                                                         cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           <  1:25394/124‑1 (MQ=255)
                                                                                                                             |                                                                                                                                
CTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016376‑2016629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑