Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,285,635	G→C	G181R (GGC→CGC)	nadB →	L‑Aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,285,635	0	G	C	100.0%	102.4 / NA	27	G181R (GGC→CGC)	nadB	L‑Aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/13);  total (14/13)

GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGG  >  AM260479/3285505‑3285761                                                                                                                                   |                                                                                                                               gcagcaGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCcgcg                                                                                                                             >  2:486259/1‑134 (MQ=255) gcagcaGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCcgcg                                                                                                                             >  2:839744/1‑134 (MQ=255)           aTGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAc                                                                                                                   >  1:535069/1‑134 (MQ=255)            tGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAcc                                                                                                                  <  2:249592/134‑1 (MQ=255)                        aCATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACg                                                                                                      <  2:1021552/134‑1 (MQ=255)                          aTCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGca                                                                                                    >  1:762597/1‑134 (MQ=255)                           tCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGca                                                                                                    >  1:869673/1‑133 (MQ=255)                                     ggAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTgg                                                                                         >  2:556612/1‑134 (MQ=255)                                        aGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCcg                                                                                      <  1:486259/134‑1 (MQ=255)                                                  gCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTg                                                                             <  2:762597/133‑1 (MQ=255)                                                   cAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGcc                                                                           <  2:869673/134‑1 (MQ=255)                                                     aTCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCAc                                                                                                       <  1:320152/104‑1 (MQ=255)                                                     aTCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCAc                                                                                                       >  2:320152/1‑104 (MQ=255)                                                      tCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc                                                                        <  1:871213/134‑1 (MQ=255)                                                                  ccTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTg                                                            >  1:330641/1‑134 (MQ=255)                                                                           aGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACACGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTAcaccac                                                    >  2:53905/1‑133 (MQ=255)                                                                            gCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACaccacc                                                   >  2:967863/1‑133 (MQ=255)                                                                                    tGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGc                                                                                                     >  1:657156/1‑75 (MQ=255)                                                                                    tGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGc                                                                                                     <  2:657156/75‑1 (MQ=255)                                                                                          gCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCaa                                                                      <  1:345473/100‑1 (MQ=255)                                                                                          gCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCaa                                                                      >  2:345473/1‑100 (MQ=255)                                                                                                    ctACGGCCTGTACGTACTGGACGACAGCACACGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGAc                           <  1:53905/133‑1 (MQ=255)                                                                                                                cgtacTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATgg              >  1:212148/1‑134 (MQ=255)                                                                                                                         cgacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGgcgcg     <  2:330641/134‑1 (MQ=255)                                                                                                                          gacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGcc                                                                           <  2:682155/63‑1 (MQ=255)                                                                                                                          gacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGcc                                                                           >  1:682155/1‑63 (MQ=255)                                                                                                                            cAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc                                                                            <  2:1055677/60‑1 (MQ=255)                                                                                                                            cAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc                                                                            >  1:1055677/1‑60 (MQ=255)                                                                                                                            cAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCgg  >  2:161804/1‑134 (MQ=255)                                                                                                                                   |                                                                                                                               GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGG  >  AM260479/3285505‑3285761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑