| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 2,253,193 | +C | coding (243/258 nt) | h16_A2071 → | Hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 2,253,191 | 1 | . | C | 86.7% | 45.7 / 0.7 | 15 | coding (241/258 nt) | h16_A2071 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (5/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑C‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGG > AM260479/2253058‑2253251 | cTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCaaa > 2:91125/1‑134 (MQ=255) cgcgGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCg > 2:885541/1‑134 (MQ=255) cgGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCt < 1:885541/134‑1 (MQ=255) cACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATc < 2:893849/132‑1 (MQ=255) gCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGcc < 2:206504/134‑1 (MQ=255) gcgcAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAg < 1:91125/133‑1 (MQ=255) ttCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATc > 1:670568/1‑82 (MQ=255) ttCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATc < 2:670568/82‑1 (MQ=255) tAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CACTGCGCCCGCTGGTAGCGGCCGAGATCGCGCa < 1:692461/78‑1 (MQ=255) tAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CACTGCGCCCGCTGGTAGCGGCCGAGATCGCGCa > 2:692461/1‑78 (MQ=255) ttCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCgaga < 1:315205/55‑1 (MQ=255) ttCGCTCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCgaga > 2:315205/1‑55 (MQ=255) tCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCa < 1:485192/57‑1 (MQ=39) tCCGCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCa > 2:485192/1‑57 (MQ=39) gCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAgg > 1:296542/1‑81 (MQ=255) gCAGCCGGTGCAGTGGCAAACC‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAgg < 2:296542/81‑1 (MQ=255) | CTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑C‑CTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGG > AM260479/2253058‑2253251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |