Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,983,831	C→T	D23N (GAC→AAC)	ntrX ←	response regulator containing a Fis‑type DNA‑binding domain, NtrX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,983,831	0	C	T	100.0%	133.5 / NA	35	D23N (GAC→AAC)	ntrX	response regulator containing a Fis‑type DNA‑binding domain, NtrX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

TTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGG  >  AM260479/3983704‑3983936                                                                                                                                |                                                                                                          ttGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAg                                                                                                     >  2:522822/1‑134 (MQ=255)             aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                         >  1:625539/1‑134 (MQ=255)             aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                         >  2:464898/1‑134 (MQ=255)                  aTCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCt                                                                                    >  1:854000/1‑134 (MQ=255)                                  cAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAttc                                                                     <  1:457932/133‑2 (MQ=255)                                               gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                        >  1:453848/1‑133 (MQ=255)                                               gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                        >  2:493345/1‑133 (MQ=255)                                                caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                        >  1:191153/1‑132 (MQ=255)                                                caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                      <  1:522822/134‑1 (MQ=255)                                                  cGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAgg                                                    <  2:854000/134‑1 (MQ=255)                                                   gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                       >  1:434707/1‑82 (MQ=255)                                                   gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                       <  2:434707/82‑1 (MQ=255)                                                              tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAttt                                                                     <  2:642775/105‑1 (MQ=255)                                                              tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAttt                                                                     >  1:642775/1‑105 (MQ=255)                                                                cccGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATAc                                       <  1:514727/133‑1 (MQ=255)                                                                cccGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACt                                      <  2:221131/134‑1 (MQ=255)                                                                     cgcgcgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTcccc                                 <  1:464898/134‑1 (MQ=255)                                                                           gTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTaa                           >  1:285361/1‑134 (MQ=255)                                                                                  cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                    <  2:221843/134‑1 (MQ=255)                                                                                  cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                    <  2:625539/134‑1 (MQ=255)                                                                                  cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                    <  1:493345/134‑1 (MQ=255)                                                                                        tGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                       >  1:916575/1‑45 (MQ=255)                                                                                        tGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                       <  2:916575/45‑1 (MQ=255)                                                                                         gCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGc               <  2:453848/132‑1 (MQ=255)                                                                                          cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGtt            >  1:945838/1‑134 (MQ=255)                                                                                                      cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCgg  <  2:191153/132‑1 (MQ=255)                                                                                                      cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg   <  2:285361/131‑1 (MQ=255)                                                                                                                aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                              <  2:946674/78‑1 (MQ=255)                                                                                                                aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                              >  1:946674/1‑78 (MQ=255)                                                                                                                aCCACGTGGCCTTCGTTGCTGAGGATCTACGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAc                     <  2:72983/103‑1 (MQ=255)                                                                                                                aCCACGTGGCCTTCGTTGCTGAGGATCTACGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAc                     >  1:72983/1‑103 (MQ=255)                                                                                                                  cACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                      <  2:802600/68‑1 (MQ=255)                                                                                                                  cACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                      >  1:802600/1‑68 (MQ=255)                                                                                                                         ccTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                        <  1:945382/75‑1 (MQ=255)                                                                                                                         ccTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                        >  2:945382/1‑75 (MQ=255)                                                                                                                                |                                                                                                          TTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGG  >  AM260479/3983704‑3983936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑