| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 619,859 | G→T | H108N (CAC→AAC) | acrA1 ← | Cation/multidrug efflux system, mebrane‑fusion component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 619,859 | 0 | G | T | 100.0% | 90.4 / NA | 24 | H108N (CAC→AAC) | acrA1 | Cation/multidrug efflux system, mebrane‑fusion component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/11); total (13/11) | |||||||||||
GGAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTGGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGC > AM260479/619730‑619946 | ggAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 2:800343/1‑133 (MQ=255) gAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGTAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 1:60533/1‑132 (MQ=255) cgGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 1:429741/134‑1 (MQ=255) ggCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGTAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATc < 2:60533/132‑1 (MQ=255) ggCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCa > 1:70441/1‑134 (MQ=255) ggCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCa > 2:288474/1‑134 (MQ=255) gCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa > 1:451916/1‑106 (MQ=255) gCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 2:451916/106‑1 (MQ=255) gACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGt < 1:288474/132‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGtt > 1:215821/1‑71 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGtt < 2:215821/71‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGt < 1:1056419/70‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGt > 2:1056419/1‑70 (MQ=255) gcagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCgggg < 2:70441/134‑1 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGt < 1:166859/99‑1 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGt > 2:166859/1‑99 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTgg > 1:767557/1‑101 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTgg < 2:767557/101‑1 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAgc < 2:364117/119‑1 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAgc > 1:364117/1‑119 (MQ=255) cagcGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGgc > 1:483368/1‑134 (MQ=255) ccGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGgc > 1:216299/1‑134 (MQ=255) ccGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCt > 1:6536/1‑48 (MQ=255) ccGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCt < 2:6536/48‑1 (MQ=255) | GGAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTGGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGC > AM260479/619730‑619946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |