Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	916,512	T→C	V341A (GTG→GCG)	h16_A0836 →	predicted exporter of the RND superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	916,512	0	T	C	100.0%	197.5 / NA	50	V341A (GTG→GCG)	h16_A0836	predicted exporter of the RND superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

CAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTAC  >  AM260479/916394‑916636                                                                                                                       |                                                                                                                             cagcagATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCt                                                                                                               >  2:138843/1‑134 (MQ=255)   gcagATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTa                                                                                                             <  1:908755/134‑1 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                                         <  1:108870/114‑1 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                                         >  2:108870/1‑114 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                                     >  1:109424/1‑134 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                                     <  1:138843/134‑1 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                                     >  2:643728/1‑134 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                                     >  2:216115/1‑134 (MQ=255)           aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                                     >  1:661786/1‑134 (MQ=255)             tttATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTc                                                                                                                       >  1:197930/1‑114 (MQ=255)             tttATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTc                                                                                                                       <  2:197930/114‑1 (MQ=255)             tttATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatccc                                                                                                   >  2:974981/1‑134 (MQ=255)             tttATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatccc                                                                                                   >  2:420270/1‑134 (MQ=255)                 tCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgat                                                                                                <  1:384298/133‑1 (MQ=255)                   gTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgatcc                                                                                              <  1:216115/133‑1 (MQ=255)                     ccGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                                         >  1:360851/1‑104 (MQ=255)                     ccGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                                         <  2:360851/104‑1 (MQ=255)                          aGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa                                                                                                       >  1:42979/1‑117 (MQ=255)                          aGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa                                                                                                       <  2:42979/117‑1 (MQ=255)                          aGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATc                                                                                      >  2:947644/1‑134 (MQ=255)                            gTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATcc                                                                                     >  1:827588/1‑133 (MQ=255)                                   aGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                                   >  2:45799/1‑96 (MQ=255)                                   aGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                                   <  1:45799/96‑1 (MQ=255)                                          cGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCa                                                                      <  2:404222/134‑1 (MQ=255)                                              tCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTg                                                                             <  2:371445/123‑1 (MQ=255)                                              tCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTg                                                                             >  1:371445/1‑123 (MQ=255)                                                   cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTa                                                                                                             <  2:653248/86‑1 (MQ=255)                                                   cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTa                                                                                                             >  1:653248/1‑86 (MQ=255)                                                   cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCAc                                                                     >  2:702395/1‑126 (MQ=255)                                                   cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCAc                                                                     <  1:702395/126‑1 (MQ=255)                                                     ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc                                                           <  2:429785/134‑1 (MQ=255)                                                     ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc                                                           <  2:567370/134‑1 (MQ=255)                                                     ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc                                                           <  2:109424/134‑1 (MQ=255)                                                     ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc                                                           <  2:827588/134‑1 (MQ=255)                                                        ccgccCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg                                                         <  1:375644/133‑1 (MQ=255)                                                        ccgccCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg                                                         <  1:947644/133‑1 (MQ=255)                                                           cccGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg                                                         <  2:225396/130‑1 (MQ=255)                                                           cccGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg                                                         >  1:225396/1‑130 (MQ=255)                                                                          ccGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTc                                      <  1:420270/134‑1 (MQ=255)                                                                                                gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATtct                                                                                                         <  2:209195/45‑1 (MQ=255)                                                                                                gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATtct                                                                                                         >  1:209195/1‑45 (MQ=255)                                                                                                gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgatc                                                                                               >  1:76914/1‑55 (MQ=255)                                                                                                gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgatc                                                                                               <  2:76914/55‑1 (MQ=255)                                                                                                     cgcTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTggcg           <  2:567026/134‑1 (MQ=255)                                                                                                     cgcTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTggcg           >  1:567026/1‑134 (MQ=255)                                                                                                              ccGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTc                                      >  1:223446/1‑98 (MQ=255)                                                                                                              ccGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTc                                      <  2:223446/98‑1 (MQ=255)                                                                                                              ccGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTAc  >  2:873814/1‑134 (MQ=255)                                                                                                                   cTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGt                      <  1:727179/109‑1 (MQ=255)                                                                                                                   cTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGt                      >  2:727179/1‑109 (MQ=255)                                                                                                                       |                                                                                                                             CAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTAC  >  AM260479/916394‑916636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑