Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	245,288	T→G	L247V (TTG→GTG)	betA2 →	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	245,288	0	T	G	100.0%	99.3 / NA	26	L247V (TTG→GTG)	betA2	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/11);  total (15/11)

CTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGTTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCAC  >  AM260479/245164‑245404                                                                                                                             |                                                                                                                     cTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGcgcg                                                                                                             <  1:619312/134‑1 (MQ=255)             tGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCgg                                                                                                  >  2:997338/1‑133 (MQ=255)                 aacaaGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATTCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAAct                                                                                             >  2:532351/1‑134 (MQ=255)                 aacaaGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAAct                                                                                             >  2:805590/1‑134 (MQ=255)                      gcgcGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACtgctgc                                                                                        >  2:180523/1‑134 (MQ=255)                           aCCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcgcgcg                                                                                   >  2:922666/1‑134 (MQ=255)                            ccGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTgcgcgcgc                                                                                  <  2:468782/134‑1 (MQ=255)                                            ggcCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCgt                                                                  <  1:805590/134‑1 (MQ=255)                                            ggcCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCgt                                                                  <  1:532351/134‑1 (MQ=255)                                                                 cTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTc                                             <  1:922666/134‑1 (MQ=255)                                                                    aCCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcgcc                                          >  1:793015/1‑134 (MQ=255)                                                                    aCCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcg                                            <  1:997338/132‑1 (MQ=255)                                                                               ggCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGa                               >  1:367068/1‑134 (MQ=255)                                                                                    tGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGgtg                          >  2:829813/1‑134 (MQ=255)                                                                                       gcATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCg                       >  2:438463/1‑134 (MQ=255)                                                                                       gcATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCg                       >  1:228661/1‑134 (MQ=255)                                                                                                    aGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccg          >  1:299760/1‑134 (MQ=255)                                                                                                    aGGCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccg          >  2:837926/1‑134 (MQ=255)                                                                                                     ggCCGCCATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCccgc         <  2:228661/134‑1 (MQ=255)                                                                                                           cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcgc                                           >  2:470374/1‑94 (MQ=255)                                                                                                           cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTcgc                                           <  1:470374/94‑1 (MQ=255)                                                                                                           cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCg                >  2:765297/1‑121 (MQ=255)                                                                                                           cATGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCg                <  1:765297/121‑1 (MQ=255)                                                                                                            aTGCGGCCGGGGTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCAc  <  2:299760/134‑1 (MQ=255)                                                                                                                     gggTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCa                                                       >  2:593171/1‑72 (MQ=255)                                                                                                                     gggTGCAGGTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCa                                                       <  1:593171/72‑1 (MQ=255)                                                                                                                             |                                                                                                                     CTACCTGCACGGTGGCAACAAGCGCGACCGGGCCTGCAATGGCGGCCGCGCGCACCTGCGGGTGCTGACCGATACGCAGGCGCTGCGCATAGTCTTCGAAGGCCGCCATGCGGCCGGGGTGCAGTTGGTGCGCGAGGGCCACCGGCAACTGCTGCGCGCGCGCCGCGACGTGATCGTGTGCGCGGGCACGTTCGGCTCGCCGCAGCTGCTGATGGTGTCGGGCGTCGGCCCCGCCGCGCAC  >  AM260479/245164‑245404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑