Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,579,212	G→C	A121A (GCC→GCG)	h16_A2374 ←	Metallopeptidase, M23B subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,579,212	0	G	C	100.0%	77.7 / NA	21	A121A (GCC→GCG)	h16_A2374	Metallopeptidase, M23B subfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/10);  total (11/10)

CCGGCGCGGCGGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGC  >  AM260479/2579088‑2579328                                                                                                                             |                                                                                                                     ccGGCGCGGCGGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGc                                                                                                             <  1:574809/134‑1 (MQ=255)      gcggcgGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCAtggt                                                                                                        <  1:361296/134‑1 (MQ=255)           ggcCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCa                                                                                                   <  1:251691/134‑1 (MQ=255)                   cgggcgcggcgggcgTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGgc                                                                                           >  2:877046/1‑134 (MQ=255)                    gggcgcggcgggcgTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTTCCCGGCGCCACCGGCATGGTGGCCACGCCGGgcg                                                                                          >  1:532613/1‑134 (MQ=255)                                                    cggccgcggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATg                                                          >  2:919980/1‑134 (MQ=255)                                                        cgcggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCgca                                                      <  2:532613/134‑1 (MQ=255)                                                                                 gcCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCAc                                                              >  1:811120/1‑101 (MQ=255)                                                                                 gcCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCAc                                                              <  2:811120/101‑1 (MQ=255)                                                                                      gcgggcCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt                        <  1:877046/134‑1 (MQ=255)                                                                                                   aTCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGgttgtt           >  1:332452/1‑134 (MQ=255)                                                                                                        cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCAc                                                              <  1:8015/78‑1 (MQ=255)                                                                                                        cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCAc                                                              >  2:8015/1‑78 (MQ=255)                                                                                                        cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt                        >  1:878603/1‑116 (MQ=255)                                                                                                        cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt                        <  2:878603/116‑1 (MQ=255)                                                                                                             ccTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGc  >  1:170519/1‑133 (MQ=255)                                                                                                                         ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGAt                                                           <  1:1004739/64‑1 (MQ=255)                                                                                                                         ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGAt                                                           >  2:1004739/1‑64 (MQ=255)                                                                                                                         ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGtt    >  2:230007/1‑119 (MQ=255)                                                                                                                         ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGtt    <  1:230007/119‑1 (MQ=255)                                                                                                                          cGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCcggc                                                                   >  1:236838/1‑55 (MQ=39)                                                                                                                             |                                                                                                                     CCGGCGCGGCGGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGC  >  AM260479/2579088‑2579328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑