Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,580,039	A→G	R155R (CGT→CGC)	h16_A2375 ←	Protein‑L‑isoaspartate carboxylmethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,580,039	0	A	G	100.0%	79.3 / NA	21	R155R (CGT→CGC)	h16_A2375	Protein‑L‑isoaspartate carboxylmethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

TGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGC  >  AM260479/2579924‑2580160                                                                                                                    |                                                                                                                          tGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATg                                                                                                         >  1:944637/1‑134 (MQ=255)     cTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATgcgcg                                                                                                     >  2:410693/1‑134 (MQ=255)                        cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt                                                                                                             <  2:897782/107‑1 (MQ=255)                        cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt                                                                                                             >  1:897782/1‑107 (MQ=255)                            cgACCTGGCTCAGCAACGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTg                                                                                    <  2:543688/128‑1 (MQ=255)                            cgACCTGGCTCAGCAACGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTg                                                                                    >  1:543688/1‑128 (MQ=255)                                       aGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgca                                                                                                                       <  2:425529/82‑1 (MQ=255)                                       aGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgca                                                                                                                       >  1:425529/1‑82 (MQ=255)                                              cgGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATc                                                            <  2:944637/134‑1 (MQ=255)                                                               ccGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGAc                                                                                          <  2:155801/87‑1 (MQ=255)                                                               ccGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGAc                                                                                          >  1:155801/1‑87 (MQ=255)                                                                      cGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAgcg                                                     >  1:688779/1‑117 (MQ=255)                                                                      cGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAgcg                                                     <  2:688779/117‑1 (MQ=255)                                                                        aTCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACAGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg                                                   >  1:44402/1‑117 (MQ=255)                                                                        aTCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACAGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg                                                   <  2:44402/117‑1 (MQ=255)                                                                                     gcTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTccc                     <  1:65137/134‑1 (MQ=255)                                                                                           gcggcgCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTc                >  1:399385/1‑133 (MQ=255)                                                                                                     ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTg                                                                                    <  2:207883/55‑1 (MQ=255)                                                                                                     ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTg                                                                                    >  1:207883/1‑55 (MQ=255)                                                                                                     ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGt     >  1:272567/1‑134 (MQ=255)                                                                                                        ggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGgc  <  2:272567/134‑1 (MQ=255)                                                                                                                    |                                                                                                                          TGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGC  >  AM260479/2579924‑2580160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑