| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 2,608,803 | T→C | W365R (TGG→CGG) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 2,608,803 | 0 | T | C | 100.0% | 54.4 / NA | 15 | W365R (TGG→CGG) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/10); total (5/10) | |||||||||||
GTGCACCTGGAACGCCGCCTGGCCGAGAAGCGCGTCTACCCGTCGATCAACCTGAACAAGTCGGGCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCTGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACGAAGAACAACGCCGAGTTTTTCGATATGATGCGCA > AM260479/2608689‑2608914 | gTGCACCTGGAACGCCGCCTGGCCGAGAAGCGCGTCTACCCGTCGATCAACCTGAACAAGTCGGGCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCAt < 1:570528/134‑1 (MQ=255) ccGAGAAGCGCGTCTACCCGTCGATCAACCTGAACAAGTCGGGCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAg < 1:159006/134‑1 (MQ=255) aaCAAGTCGGGCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGc < 1:1025062/134‑1 (MQ=255) ggCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATtcatca > 1:720537/1‑109 (MQ=255) ggCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATtcatca < 2:720537/109‑1 (MQ=255) ggCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACgaagaa > 2:483035/1‑134 (MQ=255) cgAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCAtctc > 1:460257/1‑63 (MQ=255) cgAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCAtctc < 2:460257/63‑1 (MQ=255) gagTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACGAAGAACAACGCCGAGttttt < 2:567187/134‑1 (MQ=255) aGCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACGAAGAACAACGCCGAGTTTTTCGATATGATgcgc < 2:136099/134‑1 (MQ=255) gCCGGAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACGAAGAACAACGCCGAGTTTTTCGATATGATGCGCa < 1:483035/134‑1 (MQ=255) gAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATg < 1:375712/66‑1 (MQ=255) gAAATCCTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATg > 2:375712/1‑66 (MQ=255) ccTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTcatcat > 1:586324/1‑72 (MQ=255) ccTGCAGAAGATCCGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTcatcat < 2:586324/72‑1 (MQ=255) | GTGCACCTGGAACGCCGCCTGGCCGAGAAGCGCGTCTACCCGTCGATCAACCTGAACAAGTCGGGCACGCGCCGCGAAGAGTTGCTGATCAAGCCGGAAATCCTGCAGAAGATCTGGATCCTGCGCAAGTTCATCTCCGACATGGATGAAGTGCAGGCGATGGAATTCATCATGGACAAGATGAAGGCCACGAAGAACAACGCCGAGTTTTTCGATATGATGCGCA > AM260479/2608689‑2608914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |