Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,983,831	C→T	D23N (GAC→AAC)	ntrX ←	response regulator containing a Fis‑type DNA‑binding domain, NtrX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,983,831	0	C	T	100.0%	147.3 / NA	39	D23N (GAC→AAC)	ntrX	response regulator containing a Fis‑type DNA‑binding domain, NtrX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

AGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTT  >  AM260479/3983710‑3983947                                                                                                                          |                                                                                                                     aGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGAtctc                                                                                                          >  1:15766/1‑134 (MQ=255)    gTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCga                                                                                                       <  1:279788/134‑1 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                    >  2:148058/1‑134 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                    <  2:1087752/134‑1 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                    >  2:956054/1‑134 (MQ=255)                 tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                           >  1:349148/1‑133 (MQ=255)                 tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                           >  1:957100/1‑133 (MQ=255)                 tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                           >  2:366732/1‑133 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                        <  1:130928/96‑1 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                        >  2:130928/1‑96 (MQ=255)                                             gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                  <  1:773440/82‑1 (MQ=255)                                             gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                  >  2:773440/1‑82 (MQ=255)                                             gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                              >  1:955652/1‑134 (MQ=255)                                                   gggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                                         <  1:458901/133‑1 (MQ=255)                                                   gggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                                         <  2:15766/133‑1 (MQ=255)                                                       gTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCAt                                                    <  1:148058/134‑1 (MQ=255)                                                        tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                        >  1:34521/1‑81 (MQ=255)                                                        tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                        <  2:34521/81‑1 (MQ=255)                                                        tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                   <  1:324001/134‑1 (MQ=255)                                                                   cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATAc                                                  >  2:400437/1‑124 (MQ=255)                                                                   cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATAc                                                  <  1:400437/124‑1 (MQ=255)                                                                   cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCctct                                         >  2:1053643/1‑133 (MQ=255)                                                                   cgGTATCCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCctct                                         >  1:508313/1‑133 (MQ=255)                                                                            cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                               <  2:349148/134‑1 (MQ=255)                                                                                  tGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCg                         >  1:559929/1‑134 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTc                                                                               <  2:930952/78‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTc                                                                               >  1:930952/1‑78 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                              >  2:978146/1‑95 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                              <  1:978146/95‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                   >  1:867118/1‑106 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                   <  2:867118/106‑1 (MQ=255)                                                                                         cgTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCggg                  <  1:784482/134‑1 (MQ=255)                                                                                               ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGa            >  1:718345/1‑134 (MQ=255)                                                                                               ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGa            >  1:415329/1‑134 (MQ=255)                                                                                                cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg              <  1:956054/131‑1 (MQ=255)                                                                                                cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg              <  2:415329/131‑1 (MQ=255)                                                                                                cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg              <  2:559929/131‑1 (MQ=255)                                                                                                cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg              <  2:955652/131‑1 (MQ=255)                                                                                                          aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCAttgtt  >  1:102456/1‑133 (MQ=255)                                                                                                                          |                                                                                                                     AGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTT  >  AM260479/3983710‑3983947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑