Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	153,689	+G	intergenic (‑67/+93)	h16_B0133 ← / ← h16_B0134	conserved hypothetical protein/outer membrane protein (porin)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	153,687	1	.	G	100.0%	180.5 / NA	46	intergenic (‑65/+95)	h16_B0133/h16_B0134	conserved hypothetical protein/outer membrane protein (porin)
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/23);  total (23/23)

GTGGTGTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑GGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGCAGGCCGGCCATG  >  AM260480/153564‑153809                                                                                                                             |                                                                                                                           gtggtgTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCat                                                                                                                    >  2:769591/1‑133 (MQ=255) gtggtgTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCat                                                                                                                    >  2:212878/1‑133 (MQ=255)   ggtgTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaa                                                                                                                 >  1:762874/1‑134 (MQ=255)    gtgtTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaat                                                                                                                >  2:104940/1‑134 (MQ=255)    gtgtTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaat                                                                                                                >  1:687712/1‑134 (MQ=255)    gtgtTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaa                                                                                                                 <  2:1028582/133‑1 (MQ=255)     tgtTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCatgaatg                                                                                                               <  2:762874/134‑1 (MQ=255)                ttCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                                                          <  1:630925/128‑1 (MQ=255)                ttCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                                                          >  2:630925/1‑128 (MQ=255)                  ctcGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTctgct                                                                                                  >  1:389578/1‑134 (MQ=255)                   tcGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTctgctg                                                                                                 >  1:544946/1‑134 (MQ=255)                       gcggggcgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAgg                                                                                             <  1:212878/134‑1 (MQ=255)                            gcgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  2:389578/134‑1 (MQ=255)                            gcgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  1:583520/134‑1 (MQ=255)                            gcgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  2:687712/134‑1 (MQ=255)                            gcgTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  2:1042833/134‑1 (MQ=255)                              gTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATgg                                                                                      >  1:216622/1‑134 (MQ=255)                              gTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATgg                                                                                      >  1:1062279/1‑134 (MQ=255)                              gTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATgg                                                                                      >  2:894220/1‑134 (MQ=255)                                  tgGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCttt                                                                                  <  2:975637/134‑1 (MQ=255)                                    gATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTtc                                                                                 <  2:504835/133‑1 (MQ=255)                                         tGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        >  1:89069/1‑121 (MQ=255)                                         tGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  2:89069/121‑1 (MQ=255)                                           gTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAg                                                                          >  1:276347/1‑133 (MQ=255)                                           gTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑gg                                                                                                                          <  2:1048178/84‑1 (MQ=255)                                           gTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑gg                                                                                                                          >  1:1048178/1‑84 (MQ=255)                                                   cACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCa                                                                 >  1:999396/1‑134 (MQ=255)                                                   cACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCa                                                                 <  2:822347/134‑1 (MQ=255)                                                              cggcgccggcATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCa                                                                             >  2:1100404/1‑111 (MQ=255)                                                              cggcgccggcATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCa                                                                             <  1:1100404/111‑1 (MQ=255)                                                                                    cAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTtc                                                                                 >  1:7138/1‑85 (MQ=255)                                                                                    cAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTtc                                                                                 <  2:7138/85‑1 (MQ=255)                                                                                    cAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGaa                                 >  2:699228/1‑133 (MQ=255)                                                                                                   cGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        >  1:814520/1‑63 (MQ=255)                                                                                                   cGCACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCAt                                                                                        <  2:814520/63‑1 (MQ=255)                                                                                                     cACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                                                          <  1:34775/43‑1 (MQ=255)                                                                                                     cACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAg                                                                                                          >  2:34775/1‑43 (MQ=255)                                                                                                     cACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAg                              <  2:931714/119‑1 (MQ=255)                                                                                                     cACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAg                              >  1:931714/1‑119 (MQ=255)                                                                                                     cACCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACg               >  1:246562/1‑134 (MQ=255)                                                                                                      aCCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGc              <  1:769591/134‑1 (MQ=255)                                                                                                      aCCGTTGGCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGc              <  1:104940/134‑1 (MQ=255)                                                                                                             gCGTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGCAggccg        <  2:276347/133‑1 (MQ=255)                                                                                                               gTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCGCCGAGCGGGCATgg                                           >  1:1052515/1‑96 (MQ=38)                                                                                                               gTGCGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCGCCGAGCGGGCATgg                                           <  2:1052515/96‑1 (MQ=38)                                                                                                                  cGGGCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGCAGGCCGGCCATg  <  1:894220/134‑1 (MQ=255)                                                                                                                    ggCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGcc                                                             <  2:498260/73‑1 (MQ=255)                                                                                                                    ggCTGTCCAGGGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGcc                                                             >  1:498260/1‑73 (MQ=255)                                                                                                                             |                                                                                                                           GTGGTGTTGGCCGTGTTCTCGGGCGGGGCGTTGTGGATCGTGGTCACTTCCACGCTCACGCCGGCGCCGGCATAGGGCGCGAACAGTTCGCTGACGACCGCACCGTTGGCGTGCGGGCTGTCCA‑GGCTTCATGAATGACCAGCGTCTGCTGCAGGGCCATGGCTTTCTCCAAAGGATGTGCCATGCCCGCTCGGCGCGGGCATGGCGTCTACGAAAAGTCAGAACTGGTGACGCAGGCCGGCCATG  >  AM260480/153564‑153809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑