Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	951,073	+G	intergenic (‑29/+230)	h16_B0838 ← / ← h16_B0839	conserved hypothetical protein/transcriptional regulator, CRP‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	951,073	1	.	G	100.0%	82.9 / NA	22	intergenic (‑29/+230)	h16_B0838/h16_B0839	conserved hypothetical protein/transcriptional regulator, CRP‑family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/11);  total (11/11)

CTGGGCCGACGGCGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGC  >  AM260480/950946‑951195                                                                                                                                 |                                                                                                                           cTGGGCCGACGGCGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTgcg                                                                                                                       <  2:538202/134‑1 (MQ=255)                                  cACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgcca                                                                                       <  2:107236/133‑1 (MQ=255)                                     gggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgccagcca                                                                                   >  2:261517/1‑134 (MQ=255)                                           ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGAc                                                                               >  2:776212/1‑132 (MQ=255)                                                     aCCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGc                                                                   >  1:942446/1‑134 (MQ=255)                                                             aCTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGtt                                                           >  1:75742/1‑134 (MQ=255)                                                                      catcggctgcatcggctgCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTc                                                  <  1:776212/134‑1 (MQ=255)                                                                       atcggctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCg                                                 >  1:177146/1‑134 (MQ=255)                                                                       atcggctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCg                                                 <  1:914145/134‑1 (MQ=255)                                                                                        cGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACg                                 <  1:970577/133‑1 (MQ=255)                                                                                         gTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcct                                       >  1:820854/1‑126 (MQ=255)                                                                                         gTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcct                                       <  2:820854/126‑1 (MQ=255)                                                                                                 cATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGc                        >  2:763762/1‑133 (MQ=255)                                                                                                   tCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCa                       >  2:1065776/1‑132 (MQ=255)                                                                                                   tCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCa                       <  1:1065776/132‑1 (MQ=255)                                                                                                      gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGtt                                                           >  1:62364/1‑93 (MQ=255)                                                                                                      gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGtt                                                           <  2:62364/93‑1 (MQ=255)                                                                                                      gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAAc                                  <  2:978991/118‑1 (MQ=255)                                                                                                      gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAAc                                  >  1:978991/1‑118 (MQ=255)                                                                                                                      gggCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAgcgc  <  1:261517/134‑1 (MQ=255)                                                                                                                       ggCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg                    >  2:550658/1‑115 (MQ=255)                                                                                                                       ggCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg                    <  1:550658/115‑1 (MQ=255)                                                                                                                                 |                                                                                                                           CTGGGCCGACGGCGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGC  >  AM260480/950946‑951195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑