Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,135,859	+C	intergenic (‑237/‑97)	h16_B1889 ← / → h16_B1890	transcriptional regulator, IclR‑family/APC transporter, AAT family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,135,857	1	.	C	100.0%	167.5 / NA	43	intergenic (‑235/‑99)	h16_B1889/h16_B1890	transcriptional regulator, IclR‑family/APC transporter, AAT family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/23);  total (20/23)

AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCC  >  AM260480/2135724‑2135976                                                                                                                                       |                                                                                                                        aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          <  2:415874/134‑1 (MQ=255) aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          >  1:415874/1‑134 (MQ=255)    gAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGTCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccc                                                                                                                      <  1:196291/134‑2 (MQ=255)     aaCAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccct                                                                                                                     >  1:623320/1‑132 (MQ=255)     aaCAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          <  2:60229/130‑1 (MQ=255)     aaCAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          >  1:60229/1‑130 (MQ=255)                                               ccGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACg                                                                            >  1:768728/1‑134 (MQ=255)                                                cGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGc                                                                           <  1:940822/134‑1 (MQ=255)                                                        aGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                    <  2:60254/84‑4 (MQ=255)                                                        aGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                    >  1:60254/1‑81 (MQ=255)                                                             aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          >  2:648778/1‑74 (MQ=255)                                                             aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          <  1:648778/74‑1 (MQ=255)                                                             aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            <  2:27411/104‑1 (MQ=255)                                                             aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            >  1:27411/1‑104 (MQ=255)                                                               aTGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAAc                                                            <  1:1090742/134‑1 (MQ=255)                                                                      gATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTAc                                                     >  2:797296/1‑134 (MQ=255)                                                                      gATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTAc                                                     >  2:135158/1‑134 (MQ=255)                                                                        tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            <  1:312543/93‑1 (MQ=255)                                                                        tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            >  2:312543/1‑93 (MQ=255)                                                                        tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTAcc                                                    >  1:624387/1‑133 (MQ=255)                                                                             cATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGc                                              >  2:520337/1‑134 (MQ=255)                                                                              aTCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                             <  1:520337/134‑1 (MQ=255)                                                                              aTCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                             <  2:768728/134‑1 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGc                                              >  1:766504/1‑132 (MQ=255)                                                                                 cctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAg                                           <  2:1102302/133‑1 (MQ=255)                                                                                  ctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                         <  2:624387/134‑1 (MQ=255)                                                                                  ctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                         <  2:363024/134‑1 (MQ=255)                                                                                    ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                         >  2:650128/1‑132 (MQ=255)                                                                                     cccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcag                                      >  1:305693/1‑134 (MQ=255)                                                                                       cGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                    <  1:932069/134‑1 (MQ=255)                                                                                       cGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                    <  2:305693/134‑1 (MQ=255)                                                                                         ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccc                                                                                                                      >  1:941640/1‑48 (MQ=255)                                                                                         ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccc                                                                                                                      <  2:941640/49‑2 (MQ=255)                                                                                         ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                    >  2:555600/1‑132 (MQ=255)                                                                                           aTTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAgg                                <  1:135158/134‑1 (MQ=255)                                                                                             tGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAAc                                                            >  1:550255/1‑104 (MQ=255)                                                                                             tGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAAc                                                            <  2:550255/104‑1 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          >  2:133370/1‑38 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          <  1:133370/38‑1 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCa                                                                                                                  >  2:801708/1‑46 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCa                                                                                                                  <  1:801708/46‑1 (MQ=255)                                                                                                   tACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          >  2:999258/1‑36 (MQ=255)                                                                                                   tACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                          <  1:999258/36‑1 (MQ=255)                                                                                                   tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaa                        <  1:797296/134‑1 (MQ=255)                                                                                                   tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaa                        <  1:650128/134‑1 (MQ=255)                                                                                                           gACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATc                 >  2:1101425/1‑133 (MQ=255)                                                                                                           gACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATc                 >  1:293352/1‑133 (MQ=255)                                                                                                             ccACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            <  2:374692/56‑1 (MQ=255)                                                                                                             ccACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                            >  1:374692/1‑56 (MQ=255)                                                                                                             ccACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                <  1:758884/68‑1 (MQ=255)                                                                                                             ccACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                >  2:758884/1‑68 (MQ=255)                                                                                                               aCGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCcaaca            <  2:766504/134‑1 (MQ=255)                                                                                                                 ggCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                <  2:182605/64‑1 (MQ=255)                                                                                                                 ggCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                >  1:182605/1‑64 (MQ=255)                                                                                                                    cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                >  1:994590/1‑61 (MQ=255)                                                                                                                    cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                <  2:994590/61‑1 (MQ=255)                                                                                                                         gCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTcc  <  2:124674/134‑1 (MQ=255)                                                                                                                                 ttACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGa                                                  >  2:1030484/1‑78 (MQ=255)                                                                                                                                 ttACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGa                                                  <  1:1030484/78‑1 (MQ=255)                                                                                                                                       |                                                                                                                        AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCC  >  AM260480/2135724‑2135976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑