Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	782,333	+A	intergenic (‑683/‑39)	h16_A0723 ← / → h16_A0724	hypothetical membrane protein/hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	782,333	1	.	A	100.0%	72.6 / NA	20	intergenic (‑683/‑39)	h16_A0723/h16_A0724	hypothetical membrane protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/9);  total (11/9)

GTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782209‑782453                                                                                                                               |                                                                                                                         gTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGa                                                                                                                   >  2:890637/1‑134 (MQ=255)         tGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGcc                                                                                                           >  1:708593/1‑134 (MQ=255)         tGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGcc                                                                                                           >  1:747997/1‑134 (MQ=255)                  gTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCaa                                                                                                  <  2:708593/134‑1 (MQ=255)                  gTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCaa                                                                                                  <  2:663115/134‑1 (MQ=255)                     ggCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGc                                                                                               <  1:824861/134‑1 (MQ=255)                     ggCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGc                                                                                               >  2:824861/1‑134 (MQ=255)                              ccAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAg                                                                                      >  1:160883/1‑134 (MQ=255)                                                aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGc                                                                    >  2:970957/1‑134 (MQ=255)                                                             cgcTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCg                                                       <  1:890637/134‑1 (MQ=255)                                                                             agaaATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGc                                       <  1:453539/134‑1 (MQ=255)                                                                                     ggCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGt                                                      >  1:375609/1‑111 (MQ=255)                                                                                     ggCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGt                                                      <  2:375609/111‑1 (MQ=255)                                                                                     ggCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGt                               <  2:465956/134‑1 (MQ=255)                                                                                     ggCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGt                               >  2:746307/1‑134 (MQ=255)                                                                                     ggCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGt                               >  1:465956/1‑134 (MQ=255)                                                                                        cGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCt                            <  2:572044/134‑1 (MQ=255)                                                                                             cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGcc                                                                                                           <  2:560504/50‑1 (MQ=255)                                                                                             cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGcc                                                                                                           >  1:560504/1‑50 (MQ=255)                                                                                                                  aTGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTgg  >  2:728864/1‑134 (MQ=255)                                                                                                                               |                                                                                                                         GTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782209‑782453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑