Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,081,436	+C	coding (437/447 nt)	h16_A0989 →	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,081,433	1	.	C	100.0%	41.1 / NA	12	G145A (GGC→GCC)	h16_A0989	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (8/4);  total (8/4)

GAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑CCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCT  >  AM260479/1081306‑1081551                                                                                                                                 |                                                                                                                       gAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑cccctg                                                                                                                  <  2:308587/134‑4 (MQ=255)          aTTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGAt                                                                                                          >  1:912212/1‑134 (MQ=255)                  ggCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATg                                                                                                  >  1:1043310/1‑134 (MQ=255)                   gcgcAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATg                                                                                                  <  1:276328/133‑1 (MQ=255)                                 cgtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGc                                                                                   <  2:912212/134‑1 (MQ=255)                                  gtcGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCgggg                                                                                    >  1:244487/1‑132 (MQ=255)                                            tgcGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGa                                                                                                           >  1:487485/1‑99 (MQ=255)                                            tgcGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGa                                                                                                           <  2:487485/99‑1 (MQ=255)                                                                  tGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGccc                                                  <  2:961225/134‑1 (MQ=255)                                                                                             tGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTgcgcc                       >  1:742525/1‑133 (MQ=255)                                                                                               cccGCGTCGAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTgcgcct                      >  1:861873/1‑131 (MQ=255)                                                                                                       gAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTAc             >  2:97336/1‑134 (MQ=255)                                                                                                                  cggcggCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCt  >  2:497685/1‑134 (MQ=255)                                                                                                                                 |                                                                                                                       GAGCTGGTGATTGCGCTGGCGCAGGCCTCGTCCGTCGACGTGCTGCGCTTCCGCGGCCTGTCCGATGCCGGGGCGCGCAGCAATGCGCGCCATGCCCGCGTCGAAGCCTCGGCCGGCGGCACGCAAGG‑CCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCT  >  AM260479/1081306‑1081551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑