Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	275,969	+C	coding (268/558 nt)	h16_A0264 ←	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	275,968	1	.	C	100.0%	73.9 / NA	20	A90G (GCC→GGC)	h16_A0264	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (10/10);  total (10/10)

GCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTC  >  AM260479/275858‑276087                                                                                                                |                                                                                                                        gCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATc                                                                                                   <  1:879501/134‑1 (MQ=255)     cTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCgaga                                                                                               >  1:964177/1‑134 (MQ=255)                   tGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTg                                                                                 >  2:449203/1‑134 (MQ=255)                              gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                  <  1:116570/106‑1 (MQ=255)                              gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                  >  2:736223/1‑106 (MQ=255)                              gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                  <  1:736223/106‑1 (MQ=255)                              gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                  >  2:116570/1‑106 (MQ=255)                                        gggCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGcggcc                                                            >  2:174199/1‑134 (MQ=255)                                                        gAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagcagcag                                             >  1:1016395/1‑133 (MQ=255)                                                          aaTAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagcagcagcag                                          <  2:964177/134‑1 (MQ=255)                                                               cTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCgtgg                                     <  2:684687/134‑1 (MQ=255)                                                                     ttGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                            <  1:844005/57‑1 (MQ=255)                                                                     ttGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                            >  2:844005/1‑57 (MQ=255)                                                                       gCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAg                              <  2:494264/133‑1 (MQ=255)                                                                        ccTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                             <  2:693839/133‑1 (MQ=255)                                                                                       tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAgcgc              <  1:174199/133‑1 (MQ=255)                                                                                                  ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagca                                                 >  1:609854/1‑87 (MQ=255)                                                                                                  ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagca                                                 <  2:609854/87‑1 (MQ=255)                                                                                                  ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTc  >  1:756686/1‑134 (MQ=255)                                                                                                  ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTc  >  2:276091/1‑134 (MQ=255)                                                                                                                |                                                                                                                        GCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTC  >  AM260479/275858‑276087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑