Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,515,018	C→G	noncoding (32/75 nt)	tRNA‑Pro (CGG) →	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,515,018	0	C	G	100.0%	136.7 / NA	36	noncoding (32/75 nt)	tRNA‑Pro (CGG)	tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/19);  total (17/19)

CGGCGATTTTCCTCGCCCTCTTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACCTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGCAACTCAT  >  AM260479/3514892‑3515143                                                                                                                               |                                                                                                                              cGGCGATTTTCCTCGCCCTCTTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGa                                                                                                                        <  1:40311/134‑1 (MQ=255)             tCGCCCTCTTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACAAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCg                                                                                                             >  2:600740/1‑133 (MQ=255)                  ctctTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGtt                                                                                                       <  2:866358/134‑1 (MQ=255)                  ctctTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGtt                                                                                                       <  2:1023873/134‑1 (MQ=255)                        cATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAAt                                                                                                  <  1:1023977/133‑1 (MQ=255)                                    aaCGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGa                                                                                     <  2:655502/134‑1 (MQ=255)                                           ctAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        <  2:387844/124‑1 (MQ=255)                                           ctAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        >  1:387844/1‑124 (MQ=255)                                                cTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTaaaa                                                                         <  2:410594/134‑1 (MQ=255)                                                               gcAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGtt                                                                                                       <  1:1062899/89‑1 (MQ=255)                                                               gcAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGtt                                                                                                       >  2:1062899/1‑89 (MQ=255)                                                               gcAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGt                                                                                                        <  2:842925/88‑1 (MQ=255)                                                               gcAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGt                                                                                                        >  1:842925/1‑88 (MQ=255)                                                                    cGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTg                                                     <  1:61182/134‑1 (MQ=255)                                                                       tCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGa                                                                                                                        >  2:98281/1‑64 (MQ=255)                                                                       tCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGa                                                                                                                        <  1:98281/64‑1 (MQ=255)                                                                       tCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        <  1:845994/96‑1 (MQ=255)                                                                       tCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        >  2:845994/1‑96 (MQ=255)                                                                       tCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGtt                                                  >  1:200/1‑134 (MQ=255)                                                                           aCGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTg                                                     >  1:562547/1‑127 (MQ=255)                                                                           aCGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTg                                                     <  2:562547/127‑1 (MQ=255)                                                                               ttGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCACGACCAGATTTAAAAGGCCGAATCAACGTCAGTTg                                                     <  2:291859/123‑1 (MQ=255)                                                                               ttGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCACGACCAGATTTAAAAGGCCGAATCAACGTCAGTTg                                                     >  1:291859/1‑123 (MQ=255)                                                                                   cAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTc                                      >  1:352644/1‑134 (MQ=255)                                                                                     gCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGa                                                                                     >  1:44759/1‑85 (MQ=255)                                                                                     gCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGa                                                                                     <  2:44759/85‑1 (MQ=255)                                                                                                       tAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        >  2:384968/1‑64 (MQ=255)                                                                                                       tAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAccc                                                                                        <  1:384968/64‑1 (MQ=255)                                                                                                             aGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGa                                                                   >  2:368158/1‑79 (MQ=255)                                                                                                             aGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGa                                                                   <  1:368158/79‑1 (MQ=255)                                                                                                                cTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGc         <  2:352644/134‑1 (MQ=255)                                                                                                                 tGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGc         >  1:903440/1‑133 (MQ=255)                                                                                                                   gTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGCAAc      >  1:225135/1‑134 (MQ=255)                                                                                                                       agCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAcccc                                                                                       >  2:46999/1‑49 (MQ=255)                                                                                                                       agCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCAcccc                                                                                       <  1:46999/49‑1 (MQ=255)                                                                                                                       agCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGCAACTCAt  >  1:1016664/1‑134 (MQ=255)                                                                                                                               |                                                                                                                              CGGCGATTTTCCTCGCCCTCTTCCATTCCCCAAAAAACGCTGCTAAACTTACGTTCTTTGCTGCAGACGGTCAGACGTTTGCCAGCAAACCAAATCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACCTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTCACCCCGACCAGATTTAAAAGGCCGAATCAACGTCAGTTGGTTCGGCCTTTTGTCTTTCTGCCCCCCCTCCCCGATCCTCCGGCAACTCAT  >  AM260479/3514892‑3515143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑