Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,983,831	C→T	D23N (GAC→AAC)	ntrX ←	response regulator containing a Fis‑type DNA‑binding domain, NtrX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,983,831	0	C	T	100.0%	175.1 / NA	45	D23N (GAC→AAC)	ntrX	response regulator containing a Fis‑type DNA‑binding domain, NtrX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

TTCCTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACT  >  AM260479/3983700‑3983951                                                                                                                                    |                                                                                                                         ttCCTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGc                                                                                                                        >  2:190116/1‑134 (MQ=255)                 aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                      <  2:802057/120‑1 (MQ=255)                 aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                      >  1:802057/1‑120 (MQ=255)                 aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                        >  2:622839/1‑134 (MQ=255)                      aTCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCt                                                                                                   >  2:82841/1‑134 (MQ=255)                           tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                               >  2:782576/1‑133 (MQ=255)                                ggCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTcc                                                                                         <  2:606698/134‑1 (MQ=255)                                        gATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGt                                                                                 >  1:31293/1‑134 (MQ=255)                                                   gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAt                                                                                      >  2:227688/1‑118 (MQ=255)                                                   gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAt                                                                                      <  1:227688/118‑1 (MQ=255)                                                         ggTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATc                                                                <  2:31293/134‑1 (MQ=255)                                                              ggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGagcag                                                                                                     >  1:188919/1‑92 (MQ=255)                                                              ggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGagcag                                                                                                     <  2:188919/92‑1 (MQ=255)                                                              ggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGc                                                           <  1:782576/134‑1 (MQ=255)                                                                  tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                                             <  1:12554/128‑1 (MQ=255)                                                                  tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                                             >  2:12554/1‑128 (MQ=255)                                                                  tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                       <  2:287021/134‑1 (MQ=255)                                                                  tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                       <  2:707824/134‑1 (MQ=255)                                                                  tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                       <  1:190116/134‑1 (MQ=255)                                                                    cccGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACt                                                     <  2:245514/134‑1 (MQ=255)                                                                      cgccgcGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTc                                                   >  1:257609/1‑134 (MQ=255)                                                                       gccgcGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTcc                                                  >  2:179534/1‑134 (MQ=255)                                                                                      cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                                   <  1:179534/134‑1 (MQ=255)                                                                                          cTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                     <  1:176827/96‑1 (MQ=255)                                                                                          cTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                     >  2:176827/1‑96 (MQ=255)                                                                                              cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                            <  1:831530/53‑1 (MQ=255)                                                                                              cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                            >  2:831530/1‑53 (MQ=255)                                                                                              cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                     <  1:534960/92‑1 (MQ=255)                                                                                              cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                     >  2:534960/1‑92 (MQ=255)                                                                                                       ctcCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg                  >  1:961899/1‑134 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                                  >  2:819826/1‑84 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                                  <  1:819826/84‑1 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                                   <  2:1097417/115‑1 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                                   >  1:1097417/1‑115 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGa                >  1:937971/1‑134 (MQ=255)                                                                                                         ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGa                >  1:652380/1‑134 (MQ=255)                                                                                                               gTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCa                                                                               <  2:235123/65‑1 (MQ=255)                                                                                                               gTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCa                                                                               >  1:235123/1‑65 (MQ=255)                                                                                                                    aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCg                                                                                  <  2:654326/57‑1 (MQ=255)                                                                                                                    aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCg                                                                                  >  1:654326/1‑57 (MQ=255)                                                                                                                    aCCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCAttgtt      >  1:864925/1‑133 (MQ=255)                                                                                                                     ccACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGa    <  2:542765/134‑1 (MQ=255)                                                                                                                       aCGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACt  <  1:622839/134‑1 (MQ=255)                                                                                                                              cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCggg                      <  1:925721/107‑1 (MQ=255)                                                                                                                              cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCggg                      >  2:925721/1‑107 (MQ=255)                                                                                                                                    |                                                                                                                         TTCCTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACT  >  AM260479/3983700‑3983951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑