Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 500,512 G→A W23* (TGG→TAG)  h16_A0473 → ABC‑type transporter, permease component: PAAT family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479500,5120GA100.0% 142.0 / NA 37W23* (TGG→TAG) h16_A0473ABC‑type transporter, permease component: PAAT family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

TGCCGTTGACAGCAGTGTGAAACGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTGGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCGGGCATCGCCGCCA  >  AM260479/500384‑500640
                                                                                                                                |                                                                                                                                
tGCCGTTGACAGCAGTGTGAAACGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAgatga                                                                                                                             >  1:573655/1‑134 (MQ=255)
  ccGTTGACAGCAGTGTGAAACGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAgatgat                                                                                                                            <  1:339100/133‑1 (MQ=255)
      tGACAGCAGTGTGAAACGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCgg                                                                                                                       >  2:237275/1‑134 (MQ=255)
                   aaaCGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAAc                                                                                                          >  1:144431/1‑134 (MQ=255)
                         aaGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGc                                                                                                    <  1:237275/134‑1 (MQ=255)
                          aGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGcc                                                                                                   >  1:231/1‑134 (MQ=255)
                             cATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTg                                                                                                >  2:529646/1‑134 (MQ=255)
                               tGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTggg                                                                                              <  2:573655/134‑1 (MQ=255)
                                       cGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGt                                                                                      <  2:144431/134‑1 (MQ=255)
                                       cGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGt                                                                                      >  2:647033/1‑134 (MQ=255)
                                         tttCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGc                                                                                                        <  2:730793/114‑1 (MQ=255)
                                         tttCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGc                                                                                                        >  1:730793/1‑114 (MQ=255)
                                                    ggACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGc                                                                         <  2:535890/134‑1 (MQ=255)
                                                         cAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTgg                                                                    <  2:231/134‑1 (MQ=255)
                                                          aaCATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGt                                                                   >  2:525604/1‑134 (MQ=255)
                                                           aCATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAGCAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGt                                                                   >  2:297781/1‑133 (MQ=255)
                                                           aCATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAgatga                                                                                                                             <  2:946068/75‑1 (MQ=255)
                                                           aCATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAgatga                                                                                                                             >  1:946068/1‑75 (MQ=255)
                                                           aCATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGt                                                                   >  2:961824/1‑133 (MQ=255)
                                                                  cTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCAtcggtt                                                           <  2:790251/134‑1 (MQ=255)
                                                                        cTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGc                                                     >  2:253027/1‑134 (MQ=255)
                                                                          ggCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTc                                                   >  2:23034/1‑134 (MQ=255)
                                                                           gCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCt                                                    <  1:253027/132‑1 (MQ=255)
                                                                              ttGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCg                                               >  2:671857/1‑134 (MQ=255)
                                                                               tGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTgg                                                                                 <  1:1052477/99‑1 (MQ=255)
                                                                               tGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTgg                                                                                 >  2:1052477/1‑99 (MQ=255)
                                                                                 ggCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGtgc                                            >  1:531398/1‑134 (MQ=255)
                                                                                   cAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGtgctg                                          <  1:23034/134‑1 (MQ=255)
                                                                                      ttttCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTgcgc                                       <  1:647033/134‑1 (MQ=255)
                                                                                         tCCTCGAGCAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCAc                                     <  1:297781/133‑1 (MQ=255)
                                                                                         tCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCAc                                     <  1:948626/133‑1 (MQ=255)
                                                                                                              aCGAGACCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCg               <  1:525604/134‑1 (MQ=255)
                                                                                                                  gaCCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGActc                                                                                          >  2:135215/1‑55 (MQ=255)
                                                                                                                  gaCCTACCTGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGActc                                                                                          <  1:135215/55‑1 (MQ=255)
                                                                                                                     ctacctGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCGGGCATcg        <  1:961824/134‑1 (MQ=255)
                                                                                                                       acctGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCAt                                                                            >  1:754086/1‑64 (MQ=255)
                                                                                                                       acctGGACTAGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCAt                                                                            <  2:754086/64‑1 (MQ=255)
                                                                                                                           ggactagaTGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCAtcggttcg                                                         >  1:1113306/1‑79 (MQ=255)
                                                                                                                           ggactagaTGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCAtcggttcg                                                         <  2:1113306/79‑1 (MQ=255)
                                                                                                                           ggactagaTGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCGGGCATCGCCGCCa  >  1:36464/1‑134 (MQ=255)
                                                                                                                           ggactagaTGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCGGGCATCGCCGCCa  >  2:982296/1‑134 (MQ=255)
                                                                                                                                |                                                                                                                                
TGCCGTTGACAGCAGTGTGAAACGGAAGGCATGTCCTTCCGTTTCTTTTTGAGGACGCAACATGAACTACAACTGGCATTGGGCAGTTTTCCTCGAACAGGCCGCCCAGAACGAGACCTACCTGGACTGGATGATCTCGGGCCTGAAGGTAACGCTCGCCCTGGGACTCTCGTCCTGGGTCATCGCCCTGGTCATCGGTTCGGTGCTCGGCGTGCTGCGCACCGTGCCCAACAAGTGGCTGGCGGGCATCGCCGCCA  >  AM260479/500384‑500640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑