Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 293,407 T→A Y759N (TAC→AAC)  flhF → Flagellar GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480293,4070TA100.0% 132.1 / NA 35Y759N (TAC→AAC) flhFFlagellar GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/18);  total (17/18)

GGCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGC  >  AM260480/293286‑293523
                                                                                                                         |                                                                                                                    
ggCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGcc                                                                                                          <  1:252314/134‑1 (MQ=255)
                                         tGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAg                                                                 <  2:1021726/134‑1 (MQ=255)
                                          gcgcCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGt                                                                >  2:699081/1‑134 (MQ=255)
                                             ccAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGTAGTGAg                                                             >  2:552546/1‑134 (MQ=255)
                                              cAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                            <  2:1111803/134‑1 (MQ=255)
                                              cAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                            <  1:699081/134‑1 (MQ=255)
                                                   cGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                <  1:939456/109‑1 (MQ=255)
                                                   cGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                >  2:939456/1‑109 (MQ=255)
                                                       ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                              <  2:326097/107‑1 (MQ=255)
                                                       ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                              >  1:1029693/1‑107 (MQ=255)
                                                       ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                              >  1:326097/1‑107 (MQ=255)
                                                       ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                              <  2:1029693/107‑1 (MQ=255)
                                                         ggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                 >  2:693130/1‑134 (MQ=255)
                                                          gtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATg                                                >  1:525696/1‑134 (MQ=255)
                                                              cggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                 <  2:216286/129‑1 (MQ=255)
                                                              cggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                 >  1:216286/1‑129 (MQ=255)
                                                                                     aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCt                                                       >  1:612125/1‑100 (MQ=255)
                                                                                     aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCt                                                       <  2:612125/100‑1 (MQ=255)
                                                                                     aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                     >  1:161144/1‑134 (MQ=255)
                                                                                          ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCAccg                                                                                                 >  1:473290/1‑53 (MQ=255)
                                                                                          ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCAccg                                                                                                 <  2:473290/53‑1 (MQ=255)
                                                                                            gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGc                                                                                           <  1:460134/57‑1 (MQ=255)
                                                                                            gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGc                                                                                           >  1:435677/1‑57 (MQ=255)
                                                                                            gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGc                                                                                           <  2:435677/57‑1 (MQ=255)
                                                                                            gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGc                                                                                           >  2:460134/1‑57 (MQ=255)
                                                                                                    gCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCac       <  1:289398/133‑1 (MQ=255)
                                                                                                     cATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGTAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga     <  1:552546/134‑1 (MQ=255)
                                                                                                     cATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga     <  2:525696/134‑1 (MQ=255)
                                                                                                       ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacgacg   <  1:820823/134‑1 (MQ=255)
                                                                                                       ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacgacg   >  2:820823/1‑134 (MQ=255)
                                                                                                        tGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGAcgc  >  1:581793/1‑134 (MQ=255)
                                                                                                              aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAg                                          <  2:1042107/88‑1 (MQ=255)
                                                                                                              aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAg                                          >  1:1042107/1‑88 (MQ=255)
                                                                                                              aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCac       >  1:807728/1‑123 (MQ=255)
                                                                                                              aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCac       <  2:807728/123‑1 (MQ=255)
                                                                                                                         |                                                                                                                    
GGCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGC  >  AM260480/293286‑293523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑