Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,167,791	T→C	intergenic (+238/‑365)	h16_A1070 → / → cyoA1	acyl dehydratase/bo3‑type quinol oxidase, subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,167,791	0	T	C	100.0%	176.0 / NA	45	intergenic (+238/‑365)	h16_A1070/cyoA1	acyl dehydratase/bo3‑type quinol oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

ATGCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTATGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGCCGG  >  AM260479/1167666‑1167896                                                                                                                              |                                                                                                          aTGCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTgc                                                                                                    <  1:1015524/133‑1 (MQ=255)                      gcgGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCgcgc                                                                              >  1:944991/1‑134 (MQ=255)                              gtgCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           <  1:1003506/129‑1 (MQ=255)                              gtgCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           >  2:1003506/1‑129 (MQ=255)                              gtgCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           >  2:634841/1‑129 (MQ=255)                              gtgCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           <  1:634841/129‑1 (MQ=255)                                 cGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAg                                                                   <  2:402078/134‑1 (MQ=255)                                   tCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAgg                                                                  >  2:1044360/1‑133 (MQ=255)                                   tCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGc                                                                 <  2:484140/134‑1 (MQ=255)                                            cGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        <  2:944991/134‑1 (MQ=255)                                                        cAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        >  1:445445/1‑122 (MQ=255)                                                        cAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        <  2:445445/122‑1 (MQ=255)                                                               ttCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACcgc                                                                                >  1:268606/1‑91 (MQ=255)                                                               ttCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACcgc                                                                                <  2:268606/91‑1 (MQ=255)                                                               ttCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCg                                     >  2:1091689/1‑134 (MQ=255)                                                               ttCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCg                                     >  1:24708/1‑134 (MQ=255)                                                                       cGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        <  1:664276/107‑1 (MQ=255)                                                                       cGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        >  2:664276/1‑107 (MQ=255)                                                                             gTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        <  2:484020/101‑1 (MQ=255)                                                                             gTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        >  1:484020/1‑101 (MQ=255)                                                                             gTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTAc                       <  1:556746/134‑1 (MQ=255)                                                                             gTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTAc                       <  1:661688/134‑1 (MQ=255)                                                                                       aaTGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCt                         >  2:177779/1‑122 (MQ=255)                                                                                       aaTGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCt                         <  1:177779/122‑1 (MQ=255)                                                                                       aaTGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGccgccg             >  2:802665/1‑134 (MQ=255)                                                                                           ttgcgttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAAc                                                                                   >  2:9889/1‑60 (MQ=255)                                                                                           ttgcgttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAAc                                                                                   <  1:9889/60‑1 (MQ=255)                                                                                                ttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGcc    >  2:620967/1‑134 (MQ=255)                                                                                                 tgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGCCg   >  1:419636/1‑134 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAAcc                                                                                  >  2:118051/1‑54 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAAcc                                                                                  <  1:118051/54‑1 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGaa                                                                    <  2:574297/68‑1 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGaa                                                                    >  1:574297/1‑68 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGCCgg  >  1:355478/1‑134 (MQ=255)                                                                                                  gcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGCCgg  >  1:319789/1‑134 (MQ=255)                                                                                                         aGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCt                         >  2:1050991/1‑104 (MQ=255)                                                                                                         aGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCt                         <  1:1050991/104‑1 (MQ=255)                                                                                                                 ccACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           >  1:437328/1‑46 (MQ=255)                                                                                                                 ccACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTg                                                                           <  2:437328/46‑1 (MQ=255)                                                                                                                 ccACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGgccgcc              >  1:606521/1‑107 (MQ=255)                                                                                                                 ccACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGgccgcc              <  2:606521/107‑1 (MQ=255)                                                                                                                    cGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        >  1:839685/1‑62 (MQ=255)                                                                                                                    cGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                        <  2:839685/62‑1 (MQ=255)                                                                                                                    cGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAAc                                         <  2:899008/77‑1 (MQ=255)                                                                                                                    cGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAAc                                         >  1:899008/1‑77 (MQ=255)                                                                                                                              |                                                                                                          ATGCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTATGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGCCGG  >  AM260479/1167666‑1167896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑