Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,492,923	T→C	L191L (TTG→CTG)	pdhB →	dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,492,923	0	T	C	100.0%	21.3 / NA	7	L191L (TTG→CTG)	pdhB	dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/3);  total (4/3)

CACCATCAACGCCGAAGACGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGTTGCTGCTGATCCTGGAAGGCGCCGCCGCATCAGCCGCCCCGGCAGCTGCC  >  AM260479/1492793‑1492973                                                                                                                                   |                                                   cacCATCAACGCCGAAGACGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCtgc                                                 >  2:879138/1‑134 (MQ=255)      tCAACGCCGAAGACGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCtgc                                                 <  1:827557/129‑1 (MQ=255)      tCAACGCCGAAGACGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCtgc                                                 >  2:827557/1‑129 (MQ=255)                  aCGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCTGCTGCTGATCCTGGAAGgc                                >  2:969902/1‑134 (MQ=255)                                              ggCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCTGCTGCTGATCCTGGAAGGCGCCGCCGCAGCAGCCGCCCCGGCAGCTg    <  1:879138/134‑1 (MQ=255)                                                 caccaTGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCTGCTGCTGATCCTGGAAGGCGCCGCCGCAGCAGCCGCCCCGGCAGCTgcc  <  1:607979/133‑1 (MQ=255)                                                 caccaTGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGCTGCTGCTGATCCTGGAAGGCGCCGCCGCAGCAGCCGCCCCGGCAGCTgcc  >  2:607979/1‑133 (MQ=255)                                                                                                                                   |                                                   CACCATCAACGCCGAAGACGCCGTGGTGACGCTGGAGTCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAAGGCGGCGTGGTCAAGGAAGTCAAGGTCAAGGTCGGTGACAACGTCGCCGAAGGCACGTTGCTGCTGATCCTGGAAGGCGCCGCCGCATCAGCCGCCCCGGCAGCTGCC  >  AM260479/1492793‑1492973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑