Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,016,501	T→C	V678V (GTT→GTC)	h16_A1857 →	Uncharacterized protein involved in exopolysaccharide biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,016,501	0	T	C	100.0%	147.6 / NA	39	V678V (GTT→GTC)	h16_A1857	Uncharacterized protein involved in exopolysaccharide biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

GCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016378‑2016629                                                                                                                            |                                                                                                                                 gcAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTgg                                                                                                                        >  1:318225/1‑134 (MQ=255)     ccAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg                                                                                                                    <  1:972513/134‑1 (MQ=255)     ccAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg                                                                                                                    <  1:475086/134‑1 (MQ=255)              aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:246483/133‑1 (MQ=255)              aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:779194/133‑1 (MQ=255)              aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  1:481814/133‑1 (MQ=255)                        ttCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTg                                                                                                 <  2:502638/134‑1 (MQ=255)                              gcgTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacggca                                                                                                                 <  2:115759/112‑1 (MQ=255)                              gcgTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacggca                                                                                                                 >  1:115759/1‑112 (MQ=255)                                      ggCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATcgacg                                                                                    >  2:1050755/1‑133 (MQ=255)                                                aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  2:563175/1‑113 (MQ=255)                                                aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              <  1:563175/113‑1 (MQ=255)                                                aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc                                                                         >  2:308191/1‑134 (MQ=255)                                                          gCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGgcg                                                                >  2:834216/1‑133 (MQ=255)                                                               aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGcc                                                          >  2:27765/1‑134 (MQ=255)                                                                  gACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg                                                                                                                         <  1:978216/68‑1 (MQ=255)                                                                  gACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg                                                                                                                         >  2:978216/1‑68 (MQ=255)                                                                  gACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGAc                                                       <  1:819541/134‑1 (MQ=255)                                                                  gACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGAc                                                       >  2:819541/1‑134 (MQ=255)                                                                       ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTTTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  2:350626/1‑90 (MQ=255)                                                                       ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTTTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              <  1:350626/90‑1 (MQ=255)                                                                       ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc                                                  >  2:570142/1‑134 (MQ=255)                                                                                                   tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCGCGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc                      <  1:81129/134‑1 (MQ=255)                                                                                                   tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGgcgc                                                               >  2:984952/1‑93 (MQ=255)                                                                                                   tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGgcgc                                                               <  1:984952/93‑1 (MQ=255)                                                                                                           cGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa              <  1:834216/134‑1 (MQ=255)                                                                                                            gCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:209344/39‑1 (MQ=255)                                                                                                            gCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            >  1:209344/1‑39 (MQ=255)                                                                                                            gCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTAACCGGCCAc             >  1:801902/1‑134 (MQ=255)                                                                                                             cATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa              >  2:1013362/1‑132 (MQ=255)                                                                                                             cATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa              <  1:1013362/132‑1 (MQ=255)                                                                                                              aTGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           >  1:926523/1‑134 (MQ=255)                                                                                                              aTGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCt           <  1:308191/134‑1 (MQ=255)                                                                                                                 gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCa        >  1:717406/1‑134 (MQ=255)                                                                                                                  cGGGCACTGTCTTCATCGTTGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc       <  2:801902/134‑1 (MQ=255)                                                                                                                    ggCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa              >  2:11815/1‑125 (MQ=255)                                                                                                                    ggCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa              <  1:11815/125‑1 (MQ=255)                                                                                                                       aCTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTa  <  1:420390/134‑1 (MQ=255)                                                                                                                        cTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTa  >  1:103438/1‑133 (MQ=255)                                                                                                                            |                                                                                                                                 GCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016378‑2016629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑