Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,517,380	G→C	P77A (CCA→GCC)	zntA ←	putative heavy metal transporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,517,380	0	G	C	100.0%	76.0 / NA	21	P77A (CCA→GCC)	zntA	putative heavy metal transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/7);  total (14/7)

GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGGCCAGCCG  >  AM260479/2517252‑2517506                                                                                                                                 |                                                                                                                               gcggACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCAt                                                                                                                           >  1:383017/1‑134 (MQ=255)       aaaCTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAggg                                                                                                                     >  2:600536/1‑134 (MQ=255)                                  gTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAAccgccg                                                                                           <  2:786441/133‑1 (MQ=255)                                    aGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcag                                                                                        >  2:1051113/1‑134 (MQ=255)                                    aGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcag                                                                                        >  1:776964/1‑134 (MQ=255)                                                        gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                                          >  2:485561/1‑128 (MQ=255)                                                        gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                                          <  1:485561/128‑1 (MQ=255)                                                             gcgACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAGCCGCCGCAGCAGAACACCCGGCGGCTgtcgtc                                                                 >  2:248335/1‑132 (MQ=255)                                                             gcgACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTc                                                                 >  2:399090/1‑132 (MQ=255)                                                                  gggCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTcg                                                          >  1:713980/1‑134 (MQ=255)                                                                             tcgATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCgg                                               <  1:600536/134‑1 (MQ=255)                                                                             tcgATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCgg                                               <  2:383017/134‑1 (MQ=255)                                                                                            aaGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGAc                                >  1:217805/1‑134 (MQ=255)                                                                                                      cATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGcc                       <  1:1051113/133‑1 (MQ=255)                                                                                                           ccGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcagc                                                                                       <  2:612363/64‑1 (MQ=255)                                                                                                           ccGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcagc                                                                                       >  1:612363/1‑64 (MQ=255)                                                                                                                  ggTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAg          >  1:795243/1‑134 (MQ=255)                                                                                                                       gcAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGc            <  1:8144/127‑1 (MQ=255)                                                                                                                       gcAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGc            >  2:8144/1‑127 (MQ=255)                                                                                                                         aaaGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGGCCAGcc   >  1:465413/1‑134 (MQ=255)                                                                                                                          aaGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGGCCAGCcg  >  1:1030820/1‑134 (MQ=255)                                                                                                                                 |                                                                                                                               GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGGCCAGCCG  >  AM260479/2517252‑2517506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑