Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,579,212	G→C	A121A (GCC→GCG)	h16_A2374 ←	Metallopeptidase, M23B subfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,579,212	0	G	C	100.0%	79.4 / NA	21	A121A (GCC→GCG)	h16_A2374	Metallopeptidase, M23B subfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/13);  total (8/13)

CGCGGCGGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAC  >  AM260479/2579092‑2579341                                                                                                                         |                                                                                                                                  cgcggcgGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCAtgg                                                                                                                      <  2:721787/134‑1 (MQ=255)    ggcggcCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCAtggtgg                                                                                                                   >  1:301976/1‑134 (MQ=255)     gcggcCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGc                                                                                                                  >  2:737157/1‑134 (MQ=255)            cggcgggcgcggcgggcgTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCg                                                                                                           <  2:301976/134‑1 (MQ=255)                   cgcggcgggcgTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCag                                                                                                    <  2:345832/134‑2 (MQ=255)                                         gcCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCcggcgg                                                                              >  1:890576/1‑134 (MQ=255)                                             aaGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCAc                                                                           >  2:986416/1‑133 (MQ=255)                                              aGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGa                                                                         <  2:890576/134‑1 (MQ=255)                                                               gCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCg                                                        <  2:583273/134‑1 (MQ=255)                                                                   cGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCt                                                    <  1:986416/134‑1 (MQ=255)                                                                     gCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCg                                                                                         >  1:666349/1‑95 (MQ=255)                                                                     gCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCg                                                                                         <  2:666349/95‑1 (MQ=255)                                                                        tGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATc                                               <  1:737157/134‑1 (MQ=255)                                                                            ggcCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGt                                           >  1:41127/1‑134 (MQ=255)                                                                             gcCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGtt                                          <  2:524763/134‑1 (MQ=255)                                                                                 ggcgggcCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTcaca                                      >  2:863225/1‑134 (MQ=255)                                                                                              gATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGgttgt                         <  1:863225/134‑1 (MQ=255)                                                                                                         ccTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGcc              <  1:429373/134‑1 (MQ=255)                                                                                                                     ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGcc              <  1:589834/122‑1 (MQ=255)                                                                                                                     ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGcc              >  2:589834/1‑122 (MQ=255)                                                                                                                     ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc  <  1:387147/134‑1 (MQ=255)                                                                                                                         |                                                                                                                                  CGCGGCGGCCGCGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAC  >  AM260479/2579092‑2579341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑