| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260480 | 274,470 | (G)10→9 | intergenic (+18/‑626) | h16_B0246 → / → fhaB | Hypothetical protein/filamentous hemagglutinin / adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260480 | 274,461 | 0 | G | . | 93.3% | 115.8 / 1.8 | 30 | intergenic (+9/‑635) | h16_B0246/fhaB | Hypothetical protein/filamentous hemagglutinin / adhesin |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (15/13); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GGTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGAC > AM260480/274332‑274587 | ggTGGATCTGGAGGAGGGGATGGGGGGAATCTTGCTTTCCCTCGCGGGCTGGCTGGCGGATGAGAGGCATGGGTTGAGTGAGCTGGCCGTGGTGCAGGGTCGGGGATTGCAGACTGACTGACGTTTAGCggggg < 2:296016/134‑1 (MQ=255)ggTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCggggg < 2:627391/134‑1 (MQ=255)ggTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCggggg < 1:729648/134‑1 (MQ=255) aGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGc > 1:19162/1‑133 (MQ=255) tgtgGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGc > 1:823529/1‑134 (MQ=255) cttgcttTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGcccccccc < 2:892154/134‑7 (MQ=255) cGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCa > 1:35620/1‑133 (MQ=255) gCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCaaa < 1:1078588/134‑1 (MQ=255) gCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCaaa < 2:35620/134‑1 (MQ=255) gtgagCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAAc > 1:631079/1‑133 (MQ=255) cTGGCCGTGCTGCAGGGTCAGGTATTGCATACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCcgcgc > 1:161762/1‑80 (MQ=255) cTGGCCGTGCTGCAGGGTCAGGTATTGCATACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCcgcgc < 2:161762/80‑1 (MQ=255) ggCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCctcctc > 1:361161/1‑86 (MQ=255) ggCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCctcctc < 2:361161/86‑1 (MQ=255) ggCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACgg > 2:211407/1‑133 (MQ=255) gCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGc > 2:879255/1‑133 (MQ=255) gCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa < 1:741256/134‑1 (MQ=255) ctgcAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGAc < 1:879255/133‑1 (MQ=255) tgcAGGGTCAGGTATTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGc > 1:101567/1‑134 (MQ=255) aTTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCt > 1:768623/1‑50 (MQ=255) aTTGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCt < 2:768623/50‑1 (MQ=255) ttGCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTc < 2:756382/134‑1 (MQ=255) gCAGACTGACTGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCg < 1:211407/133‑1 (MQ=255) gactgactGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCaa < 1:864804/109‑1 (MQ=255) gactgactGACGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCaa > 2:864804/1‑109 (MQ=255) ctgactgaCGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa < 2:1080622/107‑1 (MQ=255) ctgactgaCGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCa > 1:1080622/1‑107 (MQ=255) actgacGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCt < 2:859725/39‑1 (MQ=255) actgacGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCt > 1:859725/1‑39 (MQ=255) tgacGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGc > 2:648900/1‑132 (MQ=255) acGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCgaga > 2:655674/1‑134 (MQ=255) acGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCgaga > 2:49516/1‑134 (MQ=255) cGTTTAGC‑GGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGAc < 2:991647/134‑1 (MQ=255) | GGTGGATCTGGAGCAGGGGATGTGGGAAAGCTTGCTTTCCCTCGCGGCCTGGCTGGCGCATGAGACGCAGGTGTTGAGTGAGCTGGCCGTGCTGCAGGGTCAGGTATTGCAGACTGACTGACGTTTAGCGGGGGGGGGGCAGCCGTGCAATCGGCTGCCGCGCGCCTCCTCGGCGCGCAGGTCAACTTCCCCGATTTCAAAGCGGTTGAACCACACGGCAAAGACGCCATCACGCCGAGTCGGGCGCAGGCGAGAC > AM260480/274332‑274587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |