Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,972,184	+C	intergenic (‑136/‑395)	h16_A3682 ← / → h16_A3683	putative methyltransferase/Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,972,182	1	.	C	100.0%	222.0 / NA	57	intergenic (‑134/‑397)	h16_A3682/h16_A3683	putative methyltransferase/Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (29/28);  total (29/28)

GTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG  >  AM260479/3972059‑3972300                                                                                                                             |                                                                                                                       gTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:17343/134‑1 (MQ=255)    cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGa                                                                                                             <  1:16430/133‑1 (MQ=255)    cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGa                                                                                                             <  1:703263/133‑1 (MQ=255)    cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  1:788308/134‑1 (MQ=255)                   ccTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACgg                                                                                              >  2:729531/1‑133 (MQ=255)                             ccGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTc                                                                                   >  1:850711/1‑134 (MQ=255)                                     ccggccgTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  1:1050352/98‑1 (MQ=255)                                     ccggccgTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  2:1050352/1‑98 (MQ=255)                                         ccgTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgc                                                                       >  2:148770/1‑134 (MQ=255)                                                 gcgcGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:955846/86‑1 (MQ=255)                                                 gcgcGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:955846/1‑86 (MQ=255)                                                          gggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAg                                                                           <  2:581103/113‑1 (MQ=255)                                                          gggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAg                                                                           >  1:581103/1‑113 (MQ=255)                                                            gatgatACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    <  1:148770/134‑1 (MQ=255)                                                                    cGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGtgtg                                             >  2:757882/1‑133 (MQ=255)                                                                     ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:691661/107‑1 (MQ=255)                                                                     ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:691661/1‑107 (MQ=255)                                                                      gTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt                                          <  1:729531/134‑1 (MQ=255)                                                                           aCCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            >  2:873043/1‑63 (MQ=255)                                                                           aCCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  1:873043/63‑1 (MQ=255)                                                                             cGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCg                                   >  1:285910/1‑134 (MQ=255)                                                                              gTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGc                                  <  2:285910/134‑1 (MQ=255)                                                                                  cacGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCg                              <  2:434323/134‑1 (MQ=255)                                                                                    cGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACg                                                                                                     >  1:143629/1‑61 (MQ=255)                                                                                    cGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACg                                                                                                     <  2:143629/61‑1 (MQ=255)                                                                                          cGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGtt                                                                                                                 >  1:191430/1‑43 (MQ=255)                                                                                          cGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGtt                                                                                                                 <  2:191430/43‑1 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:687422/38‑1 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:687422/1‑38 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGa                                                                                                             <  2:124651/40‑1 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGa                                                                                                             >  1:124651/1‑40 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            >  1:912282/1‑41 (MQ=255)                                                                                                 ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  2:912282/41‑1 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:460595/36‑1 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:460595/1‑36 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt                                          <  2:820024/105‑1 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt                                          >  1:820024/1‑105 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcg             >  1:507812/1‑134 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcg             >  2:563808/1‑134 (MQ=255)                                                                                                   ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcg             >  2:171241/1‑134 (MQ=255)                                                                                                    cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGcc                                                                         <  2:37957/73‑1 (MQ=255)                                                                                                    cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGcc                                                                         >  1:704925/1‑73 (MQ=255)                                                                                                    cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGcc                                                                         >  1:37957/1‑73 (MQ=255)                                                                                                    cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGcc                                                                         <  2:704925/73‑1 (MQ=255)                                                                                                      ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACgg                                                                                              <  1:794697/50‑1 (MQ=255)                                                                                                      ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACgg                                                                                              >  2:794697/1‑50 (MQ=255)                                                                                                      ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg                                                         <  1:512467/87‑1 (MQ=255)                                                                                                      ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg                                                         >  2:512467/1‑87 (MQ=255)                                                                                                        ccGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:353349/72‑1 (MQ=255)                                                                                                        ccGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:353349/1‑72 (MQ=255)                                                                                                         cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:1040420/71‑1 (MQ=255)                                                                                                         cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:1040420/1‑71 (MQ=255)                                                                                                         cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAtt       >  2:745586/1‑134 (MQ=255)                                                                                                         cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAtt       >  1:984924/1‑134 (MQ=255)                                                                                                              aCCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGAtg  <  2:850711/134‑1 (MQ=255)                                                                                                                      gTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg                                                         <  2:550001/71‑1 (MQ=255)                                                                                                                      gTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg                                                         >  1:550001/1‑71 (MQ=255)                                                                                                                             |                                                                                                                       GTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG  >  AM260479/3972059‑3972300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑